毛细管气相色谱法测定原人参二醇中有机溶剂残留物

Determination of residual solvents in protopanaxdiol by CGC

目的:建立原人参二醇中3种有机溶剂残留物的检测方法。方法:采用毛细管气相色谱法,色谱柱为PLOTQ毛细管柱,N,N-二甲基乙酰胺为溶解介质,FID检测器,载气为氮气,以异丙醇为内标测定原人参二醇中甲醇、乙酸乙酯、正丁醇的有机溶剂残留量。结果:本法线性关系良好,r=0.99996～0.99999,精密度RSD均小于6.0%,甲醇、乙酸乙酯和正丁醇的加样回收率为98.2%～102.2%,其RSD在1.1～2.4%。结论:该方法操作简便快速、灵敏度高,准确度好,可作为原人参二醇原料中有机溶剂残留量的测定方法。

AIM： To establish a method for detecting the residual solvents in protopanaxdiol. METHODS： Capillary GC with HP-PLOT-Q column （30 m × 0. 53 mm × 1. 0 μm） was used to determine residual in protopanaxdiol. N,N-dimethyl-acetamide as solvent media,FID detector and nitrogen as the carrier gas. RESULTS： There was a good linearity at the experimental concentration （r = 0. 999 96-0. 999 99）,the RSD of precision was less than 6. 0 % . The spotting recovery of the 3 residul,including methol,ethyl acetate and n-butanol,was in the range of 98. 2%-102. 2% ,and its’RSD was 1. 1%-2. 4% . CONCLUSION： The method is simple,accurate and of high sensitivity,can be used for the determination of residual solvents in protopanaxdiol.