HPLC法测定玫瑰花瓣中槲皮素含量

Determination of Quercetin in Rosa rugosa Petal by HPLC

目的:建立HPLC法测定玫瑰花瓣中槲皮素含量,并对两个产地玫瑰花瓣中槲皮素含量进行比较。方法:色谱柱:Symmetry Shield RP18(150mm×4.6mm,5μm);柱温:25℃;检测波长:368nm;流速:1.0mL.min-1;流动相:甲醇-0.4%磷酸(60∶40);进样量:10μL。结果:槲皮素的平均回收率为98.4%(RSD=1.78%,n=9);和田玫瑰花瓣提取物水解样中槲皮素含量为(3.05±0.090)mg.g-1(RSD=2.95%,n=3),未水解样中槲皮素含量为(0.405±0.013)mg.g-1(RSD=3.21%,n=3);昌吉玫瑰花瓣提取物水解样中槲皮素含量为(1.841±0.013)mg.g-1(RSD=1.84%,n=3),未水解样中槲皮素含量为(0.225±0.007)mg.g-1(RSD=3.11%,n=3),和田玫瑰花瓣中槲皮素含量高于昌吉玫瑰花瓣。结论:该方法简便、快速、准确,可用于玫瑰花瓣中槲皮素含量测定及药材质量控制。

Objective： To establish a HPLC method for the determination of quercetin in Rosa rugosa petal and to compare the contents of quercetin in Rosa rugosa from two sources. Methods： The determina- tion was performed on a Symmetry Shield RP18（ 150 mm ×4.6 mm, 5 μm）, and methanol-0. 4% phosphoric acid （60 ： 40） were used as mobile phase at a column temperature of 25 ℃, at detection wave- length of 368 nm, and with a flow rate of 1.0 mL.min ^-1 and an injection volume of 10 μL. Results： The average recovery of quercetin was 98.4% （ RSD = 1.78%, n = 9）. The contents of quercetin in the hydrolysis and un-hydrolysis samples from Hotan＇s Rosa rugosa petal extract were （3.05 ± 0. 090） mg .g^-1 （ RSD = 2. 95%, n = 3） and （ 0. 405 ± 0. 013） mg·g^- 1 （ RSD = 3.21%, n = 3）, respectively, and those from Changji＇s Rosa rugosa petal extract were （ 1. 841 ± 0. 013） mg-g-1 （ RSD = 1.84%, n = 3） and （0. 225 ± 0. 007） mg. g^-1 （ RSD = 3.11%, n = 3）, respectively. The contents of quercetin in the samples from Hotan were higher than those from Changji. Conclusion： This method is simple, rapid, accurate and reliable for the determination of quercetin in Rosa rugosa petal and also for the quality control of the raw material.