摘要
目的:研究shRAKC1对慢性成瘾小鼠脑组织CREBmRNA、蛋白的表达变化。方法:通过条件性位置偏爱(CPP)实验分析shRAKC1对慢性吗啡成瘾小鼠的作用;通过RT-PCR检测RACK1和CREB的mRNA在成瘾小鼠海马中的表达水平,并采用免疫组化观察RACK1和CREB蛋白的表达情况。结果:慢性成瘾组与生盐水组相比,前者海马区RACK1和CREBmRNA表达升高(p<0.05),且吗啡诱导的CPP效应增强(p<0.05),而shRAKC1组与空质粒组相比,前者由吗啡诱导的CPP诱导效应减弱(p<0.05),同时其海马区RACK1和CREBmRNA表达下降(p<0.05)。结论:干扰慢性吗啡成瘾小鼠RACK1表达,可以使CREB表达水平下调,并有抑制吗啡成瘾效应的作用。
Objective: To investigate the effects of shRACK1 on CREB in hippocampus of mice. Method: Analysing the effects of CPP in hippocampus of mice. The expression of RACKI and CREB were determined by RT-PCR and Immunohistochemistry. Result: The level of RACK1 and CREB mRNA in morphine-induced group is higher than in the saline group(p〈0. 05), morphine-indueed group's CPP is increased(p〈0. 05). And the level of RACK 1 and CREB mRNA in control plasmid group is higher than in shRACK1 group(p〈0. 05), morphine-induced group's CPP is decreased(p〈0. 05). Conclusion: shRACK1 in mice with chronic morphine dependence can decrease the expression of CREB, and it can decrease the effects of chronic morphine dependence.
出处
《四川生理科学杂志》
2010年第2期51-54,共4页
Sichuan Journal of Physiological Sciences
基金
国家自然科学基金资助(编号:30470684)