康脑液1号预处理对大鼠脑缺血再灌注病灶和GFAP表达的影响

Effects of Kangnaoye No.1 Decoction Preconditioning on Necrotic Area and Expression of GFAP after Focal Cerebral Ischemia-reperfusion in Rats

目的观察康脑液1号预处理对SD大鼠脑缺血再灌注后病灶和胶质纤维酸性蛋白(GFAP)表达的影响,探讨其对脑缺血再灌注保护作用的可能机制。方法将180只大鼠随机分为5组(各36只),假手术组、模型(缺血再灌注)组、盐酸法舒地尔注射液组、康脑液1号A组、康脑液1号B组。采用线栓法复制SD大鼠大脑中动脉梗死模型(MCAO),分别于缺血2h后再灌注1d、7d、14d。观察康脑液1号预处理对大鼠脑缺血再灌注神经功能、梗死面积和病理形态学的影响。采用免疫组化法检测缺血半暗带和病灶中心区星形胶质细胞GFAP表达的变化。结果模型组与假手术组相比,GFAP表达阳性细胞数增多;康脑液1号预处理可不同程度地降低实验性脑缺血大鼠的神经功能评分、减小梗死面积和减轻病理形态改变(P<0.05);康脑液1号各组大鼠脑组织缺血半暗带GFAP表达减少,而梗死灶中心GFAP表达却增多;康脑液1号A组与康脑液1号B组之间比较差异无统计学意义(P>0.05)。结论康脑液1号预处理可能通过调节脑缺血再灌注后GFAP表达而促进脑损伤修复及重塑,从而发挥脑保护作用。

Objective To observe the impact of Kangnaoye No.1 decoction preconditioning on necrotic area and expression of glial fibrillary acidic protein（GFAP）after the focal cerebral ischemia-reperfusion,and investigate its neuroprotection mechanism.Methods One hundred and eighty Sprague-Dawley rats were randomly divided into five groups：Shamoperation group（n=36）,model group（n=36）,fasudil hydrochloride injection group（n=36）,Kangnaoye No.1 decoction A group（n=36）,and Kangnaoye No.1 decoction B group（n=36）.Cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion（MCAO）and followed by reperfusion at 1 d,7 d,and 14 d.The neurological function,cerebral infarction size and the pathomorphology of brain tissues were studied.The expression of GFAP was tested by immunohistochemistry.Results Compared with shamoperation group,the expression of GFAP was increased markedly in model group.Compared with model group,the scores of neurological function,the cerebral infarction size were decreased,and the pathomorphological change in brain tissues were alleviated in Kangnaoye No.1 decoction group（P0.05）.Compared with model group,the expression of GFAP around the necrotic area was decreased while the expression of GFAP in the infarction area was increased in Kangnaoye No.1 decoction.There was no significant difference between Kangnaoye No.1 decoction A group and B group（P0.05）.Conclusion Kangnaoye No.1 decoction preconditioning could regulate the expression of GFAP to protect the nerve cells after the focal cerebral ischemia-reperfusion and promote the ischemic neuron to revive.