电针对非酒精性脂肪肝大鼠血脂及肝功能的影响

Infuluence of electroacupuncture on blood lipid and liver function in nonalcoholic fatty liver rats

目的:观察电针对非酒精性脂肪肝(NAFLD)大鼠血脂及肝功能变化的影响,探讨针刺治疗脂肪肝的机制。方法:33只SD大鼠随机分为正常组(11只)和(NAFLD)组﹙22只﹚。后者以高脂饲料饲养建立NAFLD大鼠模型,8周末,处死其中3只(正常对照组1只,NAFLD组2只),以验证造模成功,然后将NAFLD组进一步分为NAFLD对照组﹙10只﹚和电针治疗组﹙10只﹚,后者施以电针"治疗"。取"足三里"、"丰隆"、"三阴交"、"太冲"穴,电针参数为疏密波,频率1.6-2Hz,强度1-4mA,以下肢轻微抖动为宜。12周末处死所有动物,检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白(LDL-C)、肝指数、血清谷丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)及肝组织病理学。结果:电针治疗组大鼠的血清TC、TG、LDL-C、肝指数、血清谷ALT及AST均显著低于NAFLD模型对照组(均P<0.05),其肝脂肪变性程度亦显著减轻。结论:电针治疗能显著地降低其血脂,改善其肝功能,从而有效的治疗非酒精性脂肪肝。

Objective：To investigate the infuluence of electroacupuncture on blood lipid and liver function in nonclcoholic fatty liver rats. Methods：Thirty-three male SD rats were randomly divided into 2 groups：normal control group （n=11,fed with normal food） and non-alcoholic fatty liver model group （NAFLD） （n=23,fed with high-fat food）. Eight weeks later 1 rats were randomly selected from the normal control group and 2 rats were randomly selected from the NAFLD group and were killed to undergo pathological examination of the liver. When the establishment of experimental model of NAFLD rats was confi rmed the remaining 20 NAFLD rats were sublishment into 2 equal subgroups：NAFLD control group to be fed continuously with high-fat food ,acupuncture treatment group （to fed continuously with high-fat food）. Take Zusanli,Hong Leong,Sanyinjiao,Taichong points,electroacupuncture -spacing parameters,2Hz frequency,intensity 4mV to the appropriate vibrating slightly lower limb. By the end of the 12th week,all rats were killed to isolate the samples of test the levels of serum total cholesterol （TC）,serum triglyceride （TG）,high-density lipoprotein cholesterol （HDL-L）,low-density lipoprotein cholesterol （LDL-CH）,liver index,Valley serum ALT（ALT）,Aspartate aminotransferase （AST）,and liver pathology. Results：Treatment of rats --serum total cholesterol （TC）,serum triglyceride （TG）,low-density lipoprotein cholesterol （LDL-CH）,liver index,Valley serum ALT（ALT）,Aspartate aminotransferase （AST） were significantly lower than those of NAFLD control group （P0.05）. Hepatic steatosis was also significantly reduced too. Conclusion：Electroacupuncture can significantly lower their blood lipids,improve their liver function and thus have effective treatment for non-alcoholic fatty liver.