竹子SRAP-PCR体系的建立与优化

Establishment and optimization of SRAP-PCR reaction for bamboos

首次报道竹类植物SRAP-PCR反应体系的建立与优化。以竹叶总DNA为模板,经过大量实验,建立和优化了竹子SRAP-PCR的50μL反应体系,20 ng模板DNA,2.0 mmo1/L Mg2+,175μmol/LdNTPs,0.2μmol/L引物,1.5 UTaqDNA聚合酶,5μL10×buffer,退火温度52℃为最优反应组合。该反应条件下扩增条带清晰,多态性丰富。

By using total DNA from bamboo leaves as tested samples,the stable 50 μL reaction system was established after a large number of experiments,which is the first report of bamboo SRAP-PCR reaction system.The best combination of reaction consists of 20 ng DNA template,2.0 mmo1/L Mg2＋,175 μmol/L dNTPs,0.2 μmol/L primer,1.5 U Taq DNA polymerase,5 μL 10 × buffer,and annealing temperature of 52℃.Under this response condition,the amplified band was clear and has rich polymorphism.