内含子的识别和选择性剪切

Summary on Recognition and Alternative Splicing of Intron

真核生物内含子的一个显著特征是在很多生物中其5'和3'剪切位点的基本序列都具有相对很高的保守性,内含子从mRNA前体转录产物中的去除和伴随的外显子的连接称作mRNA前体的剪切,它是构成真核基因表达和基因调控水平的一个重要方面。这个过程由许多具有有限序列和特殊空间结构的顺式作用元件控制,由被称为剪切体的核糖核蛋白复合体来执行。以内含子的识别和由于识别造成的选择性剪切进行了综述,试图去理解造成选择性剪切的分子机理。

A notable feature of eukaryotic nuclear pre-mRNA introns is the relatively high level of conservation of the primary sequences of 5＇ and 3＇ splice sites over a great range of organisms. The removal of introns from premRNA transcripts and the concomitant ligition of exons is known as pre-mRNA splicing. It is a fundmental aspect of constitutive eukaryotic gene expression and an important level at which gene regulated. The process is governed by multiple cis-acting elements of limited sequence content and particular spatial constraints, and is executed by a dynamic ribonucleoprotein complex called spliced variant. The way of intron recognition and the reason of alternative splicing because of recongition was summarized in this paper, and the molecular mechanism of alternative splicing may be tried to understand.