大鼠后肢缺血/再灌注后肝细胞损伤及乌司他丁的保护效应

The Effect of Ulinastatin on the Liver Function after Limb Ischemia-reperfusion in Rats

目的探讨乌司他丁对大鼠后肢缺血再灌注后肝脏功能保护作用及可能机制。方法建立健康SD大鼠后肢缺血/再灌注模型,将24只健康SD雄性大鼠随机分为3组,每组8只。检测肝功能、血清肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-10浓度,电镜观察肝脏线粒体结构及光镜观察肝脏病理结构。结果肝功能指标测定,对照组、实验组较假手术组丙氨酸氨基转移酶、天冬氨酸氨基转移酶、乳酸脱氢酶升高,实验组较对照组丙氨酸氨基转移酶、天冬氨酸氨基转移酶、乳酸脱氢酶降低;炎性因子TNF-α、IL-10测定,对照组、实验组较假手术组TNF-α升高,实验组较对照组TNF-α降低,对照组、实验组较假手数组IL-10升高,实验组较对照组IL-10升高;以上各项比较差异均具有统计学意义(P<0.05)。线粒体结构:假手术组0级,对照组2～3级,实验组1级。结论乌司他丁对下肢缺血/再灌注损伤后引起的肝脏损伤具有明显的保护作用,其作用机制可能是降低促炎性因子TNF-α、提升抑制炎性因子IL-10,减轻炎性反应,保护肝细胞,减轻因下肢缺血/再灌注损伤所导致的肝脏损伤。

Objective To observe the protective effect of ulinastatin on the liver function after limb ischemia-reperfusion in rats and the potential mechanism.Methods The developments of healthy SD rat hind limb ischemia-reperfusion model,24 healthy SD male rats were randomly divided into 3 groups of 8.Detect the liver function,concentration of serum tumor necrosis factorα（TNF-α）,interleukin（IL）-10 factor,electron microscopy of liver mitochondrial structure and optical microscopy structure of the liver Pathology.Results Liver function parameters were measured,the level of ALT,AST,LDH increased in the control group,experimental group than that in sham-operated group,and the level of ALT,AST,LDH increased in the experimental group than that in the control group.The level of inflammatory cytokines such as TNF-α,IL-10 was increased in the control group,experimental group than that in sham-operated group.The level of TNF-αdecreased in experimental group than that in the control group.The level of IL-10 increased in control group,experimental group than that in sham-operated group,the level of IL-10 increased in experimental group than that in sham-operated group.There were significant difference all of the above（P〈0.05）.The structure of mitochondria：the sham-operated group was 0 grade,the control group was 2～3 grade,and the experimental group was 1 grade.Conclusion Ulinastatin has protective effects on the liver function after limb ischemia-reperfusion in rats.The theory was to decrease the blood serum of TNF-α and raise the blood serum of IL-10,so the inflammatory reaction is inhibited to protect the herpetic function and the ultra structures of mitochondria.