高效液相色谱法测定复方石韦薄膜衣片中苦参碱的含量

DETERMINATION OF MATRINE CONTENT IN FUFANGSHIWEI FILM COATING TABLETS BY HPLC

目的:建立高效液相色谱法测定复方石韦薄膜衣片中苦参碱含量的方法。方法:采用Kromasil KR100-5C18(4.6mm×250mm,5μm)色谱柱,流动相:乙腈-0.025M磷酸(1:24),流速:1ml/min,检测波长:220nm。结果:苦参碱在16.01μg/ml-320.40μg/ml浓度范围内线性关系良好(r=0.9999),平均加样回收率为100.64%,RSD为0.87%。结论:本文建立的方法简单、灵敏、结果准确,可用于测定复方石韦薄膜衣片中苦参碱的含量。

Objective：To establish the method for determination of matrine content in Fufangshiwei film coating tablets by HPLC.Methods：Kromasil KR100-5C18 column（4.6mm×250mm,5μm） was used,the mobile phase was acetonitrile-0.025M phosphoric acid（1：24）,flow rate was 1 ml/min,and detecting wave length was 220nm.Results：The linear range of matrine was 16.01μg/ml-320.40μg/ml,the average recovery was 100.64% and RSD was 0.87%.Conclusions：This method is simple,sensitive and accurate,and it is suitable for determination of matrine content in Fufangshiwei film coating tablets.