新型雌激素受体ERα36在张氏肝细胞和人肝癌细胞中的表达比较分析

Detection and comparison of a novel estrogen receptor alpha 36 expression in Chang's liver cells and hepatoma cells

研究新型雌激素受体ERα36在人肝细胞和肝癌细胞系中的差异表达具有重要的意义.以张氏肝(Chang Liv-er)、Caveolin-1基因沉默细胞株CAV7、人肝癌细胞系SMMC-7721和HepG2为实验材料,通过免疫印迹杂交法观察了不同细胞内ERα36蛋白的表达情况,并与典型雌激素受体亚型ERα66蛋白的表达进行了比较.结果发现:(1)几种细胞中均有ERα36蛋白的表达;(2)与张氏肝细胞相比,Caveolin-1基因沉默时ERα36表达明显增加(P<0.01);(3)ERα36在SMMC-7721中表达水平较低,而在HepG2细胞中表达水平较高,与ERα66和Caveolin-1的表达水平相关.提示新型雌激素受体ERα36可能参与Caveolin-1介导的雌激素信号转导,而在肝细胞的恶性增殖过程中发挥一定的作用.

Study on the expression of a novel estrogen receptor ERα36 and comparision of the expression differences in Hepatocyte and Hepatoma Cell Lines has important significance.In this experiment,normal Chang Liver,Caveolin-1 gene silenced cell line CAV7,human liver cancer cell lines SMMC-7721 and HepG2 were cultured and Western blot was performed to detect the expression of ERα36.Then the expression differences between ERα36 and ERα66 were compared respectively.Result：（1） ERα36 is expressed in all the liver cell lines detected.（2）Compared with the normal liver cell line Chang Liver,the expression of ERα36 increased significantly in CAV7 after Caveolin-1 expression was down regulated（P0.01）.（3）Compared with Chang Liver cell line,ERα36 is low expressed in SMMC-7721,but highly expressed in HepG2.ERα36 expression was closely related to the expression levels of ERα66 and Caveolin-1.All these results indicate that ERα36 may participate in the estrogen signal pathway mediated by Caveolin-1 and plays an important role in the process of hepatocyte proliferation and transformation.