乙型肝炎患者PreS1抗原与HBV-DNA的相关性分析

Analysis of correlation between the PreS1-Ag and HBV-DNA of the patients with hepatitis B

目的探讨乙型肝炎患者前S1抗原(PreS1)抗原与乙肝病毒脱氧核糖核酸(HBV-DNA)的关系。方法对150例乙型肝炎患者标本用实时荧光定量聚合酶链反应(RT-PCR)检测HBV-DNA,用酶联免疫吸附试验(ELISA)检测PreS1抗原。结果 150例乙型肝炎患者血清中,HBV-DNA阳性91例,总检出率为60.7%(91/150),PreS1抗原阳性94例,总检出率为62.7%(94/150),其差异无统计学意义(χ2=0,P=0.566>0.05),HBV-DNA与PreS1抗原具有较好的相关性。结论 HBV-DNA与PreS1抗原具有较好的一致性,故PreS1抗原可作为判断HBV感染与复制的一项新指标,但二者的检出率并不完全相同,提示它们所表达的意义并不完全相同,各自具有独立的临床意义,故联合检测HBV-DNA与PreS1抗原更具有临床意义。

Objective To investigate the relationship between the the Pre-S1 antigen （PreS1-Ag） and HBVDNA of the patients with hepatitis B. Methods HBV DNA of the specimens of 150 cases of hepatitis B patients were detected by means of real-time quantitative-PCR （RT-PCR） while the PreS1 AG was tested by enzyme linked immunosorbent assay （ELISA）. Results Among the 150 cases of serum hepatitis B patients,the positive rate of HBV-DNA in 91 cases was 60.7% （91/150） ,and the positive rate of the PreS1-Ag in 94 cases was 62.7% （94/150）. The difference was not statistically significant （X^2=0, P= 0. 566〉0.05）, HBV-DNA and PreS1-Ag have a good correlation. Conclusion Since HBV-DNA and HBV preS1 Ag have a good consistency,PreS1-Ag could be used as a new index of HBV infection and replication. But both the detection rate is not exactly the same,suggesting that they are of no same significance in the expression, with each being of independently clinical significance. So the joint detection of HBV-DNA and HBV preS1-Ag would be of more clinical significance.