摘要
目的观察阿拉瑞林对HEC-1B裸鼠移植瘤的抑制作用及对血管内皮生长因子(VEGF)表达的影响。方法将HEC-1B荷瘤鼠随机分为5组:阴性对照组、阿拉瑞林低、中、高剂量组和阳性对照(5-Fu)组。30d后处死裸鼠,称取移植瘤质量,并观察抑瘤率及用免疫组化SABC法检测移植瘤VEGF的表达。结果阿拉瑞林组和5-Fu组平均瘤质量和抑瘤率与阴性对照组比较,差异均有统计学意义(P<0.05或P<0.01)。低、中剂量阿拉瑞林组VEGF阳性率与阴性对照组比较,差异有统计学意义(P<0.05);高剂量阿拉瑞林组、5-Fu组VEGF阳性率与阴性对照组比较,差异有统计学意义(P<0.01)。结论阿拉瑞林有一定的抑制子宫内膜癌生长的作用,且不良反应轻微,可能通过对VEGF的表达调控来实现。
Objective To observe the effect of alarelin in inhibiting the growth of transplanting HEC-1B cells in nude mice and the expression of VEGF. Methods Nude mice with transplanted HEC-1B tumor were randomly divided into five groups：Negative control group,alarelin low,middle and high-dose group and positive control（5-Fu） group. After 30 days,the nude mice were executed,then the tumor was weighed,the inhibitive rates of the tumor in nude mice was observed and immu-nohistochemistry with SABC staining method were used to detect the expression of VEGF. Results Average tumor quality and inhibition rate of alarelin group and 5-Fu group compared with that of negative control group,the differences were statistically significant（P 0. 05 or P 0. 01）. VEGF-positive rate of alarelin low,middle-dose group compared with the negative control group,the difference was statistically significant（P 0. 05）;VEGF-positive rate of alarelin high-dose group and 5-Fu group compared with the negative control group,the difference was statistically significant（ P 0. 01）. Conclusion Alarelin has good effect in inhibiting the growth of endometrial cancer by regulating VEGF expression.
出处
《临床合理用药杂志》
2010年第9期49-50,共2页
Chinese Journal of Clinical Rational Drug Use
