棕榈油酸甲酯对朱砂叶螨生物活性的影响

Acarocidal Activity of Methyl Cis-9-hexadecenoate Against Tetranychus cinnabarinus

为了研究棕榈油酸甲酯对朱砂叶螨生物活性的影响,采用玻片浸渍法和叶片残毒法测定棕榈油酸甲酯对害螨的生物活性,并采用生化方法对其体内几种酶的生物活性进行测定。结果表明,棕榈油酸甲酯具有较强的触杀和产卵抑制作用,质量浓度为5 mg/mL时,对朱砂叶螨的雌成螨和卵24 h后的校正死亡率分别达到79.82%,76.34%,LC50分别为1.853 4 mg/mL和2.213 6 mg/mL;雌成螨24 h后产卵抑制率为79.45%,驱避率为95.85%。棕榈油酸甲酯处理朱砂叶螨后,螨体内解毒酶谷胱甘肽-S-转移酶被激活,说明棕榈油酸甲酯存在对朱砂叶螨有毒的物质;而乙酰胆碱酯酶、Na+,K+-ATPase均受到不同程度的抑制,可能引起神经传递的阻断,从而导致螨体的死亡。研究表明棕榈油酸甲酯可以有效杀死朱砂叶螨,作为新型农药具有良好的开发前景。

The Methyl cis-9 hexadecenoate was investigated for its biological activity against Tetranychus cinnabarinus. Slide-dip and Leaf-residue methods were used to measure biological activity. Several enzymes in T. cinnabarinus treated with Methyl cis-9-hexadecenoate were tested. The results indicated that the bioactivities （touch kill, oviposit inhibition, repellency）of Methyl cis-9-hexadecenoate were high. The adjusted mortalities of female mite and egg were 79.82% and 76.34%. The oviposit inhibition rate and repellency rate were 79.45% and 95.85% ,respectively at the concentration of 5 mg/mL. LC50 were 1. 853 4 mg/mL and 2. 213 6 mg/mL. Methyl cis-9-hexadecenoate had strongly acaricidal activities against T. cinnabarinus. The activities of glutathione-S-transferase in T. cinnabarinus were strongly induced, but the activities of aeetylcholinease, monoamine oxidase , Na＋ , K＋-ATPase and Ca2＋-ATPase were restrained which may lead to the transmit block of nerve in mites. The result from the experiment also indicated that the Methyl cis-9-hexadecenoate had a prospect to be a new resource of botanical acaricide.