galectin-1表达下调对LST-R1细胞侵袭表型的影响

Influence on Invasive Phenotype of LST-R1 Cells Mediated by galectin-1 Downregulation

背景：前期研究发现半乳凝素（galectin）-1高表达于结直肠侧向发育型肿瘤（KST）细胞株LST-R1,由此推测其可能与LST病变的侧向生长有关.目的：探讨galectin-1表达下调对LST-R1细胞侵袭表型的影响.方法：以RNA干扰（RNAi）技术沉默LST-R1细胞的galectin-1基因,采用异质性黏附实验、同质性黏附实验、趋化运动实验、软琼脂集落形成实验比较转染galectin-1短发夹RNA（shRNA）表达载体的p-shRNA1-LST细胞、转染空载体的p-LST细胞和对照组LST-R1细胞侵袭表型的差异.结果：p-shRNA1-LST细胞的异质性黏附细胞数增加,同质性黏附细胞数减少,趋化运动能力增强,细胞集落形成数增多.结论：galectin-1表达下调可致LST-R1细胞出现侵袭相关表型,提示galectin-1可能参与了LST病变浅表性扩散特性的形成.

Background： Galeetin-1 is overexpressed in colorectal laterally spreading tumor （LST） cell line LST-R1 in prophase research, and was presumed to be associated with the laterally spreading of LST lesion. Aims： To assess the influence of galectin-1 downregulation on the invasive phenotype of LST-R1 cells. Methods： RNA interference （RNAi） was employed to knock-down the expression of galectin-1 gene in LST-R1 cells. Invasive phenotypes, including heterotypic and homotypic cell adhesion, chemotactic motion and colony formation ability in soft agar of p-shRNA1-LST cells （transfected with galectin-1 short hairpin RNA expression vector）, p-LST cells （transfected with blank vector）, and controlled LST-R1 cells were assessed. Results： p-shRNA1-LST cells had more heterotypic adhesion cells, less homotypic adhesion cells, increased chemotactic motion and colony formation ability in soft agar. Conclusions： Downregulation of galectin-1 can induce the invasive phenotype of LST-R1 cells, which indicates that galectin-1 may play a role in developing the superficial spreading specificity of LST lesion.