不对称二甲基精氨酸对人单核细胞株的活性及细胞周期的影响

Effect of Asymmetric Dimethylarginine on the Viability and Cell Cycle of Human Monocyte Cell Line

目的探讨不对称二甲基精氨酸(ADMA)对单核细胞株(THP-1)的活性及细胞周期的影响.方法体外培养THP-1并且给予不同剂量的ADMA(3.75μmol/L、7.5μmol/L、15μmol/L、30μmol/L)相互作用(24h、48h、72h)后,用改良MTT法测定THP-1的活性状态及作用(24h、48h)后流式细胞仪测定细胞生长周期.实验分为空白组、阴性对照组、不同剂量ADMA组.结果在MTT测定中与阴性对照组比较发现THP-1与ADMA共同培养下能够促进细胞的生长活性,流式细胞仪在细胞周期中检测显示ADMA能提高其细胞的S期和G2/M期峰值百分比,提示细胞处于增殖状态.结论 ADMA能够促进THP-1的生长活性及增殖状态.

Objective To explore the effect of ADMA on the activity and the viability and cell cycle of human monocytes.Methods THP-1 cells,human monocyte cell line,were cultured in vitro,and were incubated with different doses of ADMA（3.75 umol/L,7.5 umol/L,15umol/L,and 30umol/L） for different time（24 h,48 h,and 72 h）.Then the viability of cells was evaluated by using modified MTT assay,and the cell cycle was detected by flowcytometry.According to different treatments,the cells were divided into control group,negative control group and different doses of ADMA groups.Results MTT results showed that ADMA promoted the viability of THP-1 cells,compared with the negative control group.Flow cytometry results showed that ADMA increased the peak percentage of cells in S phase and G2/Mphase,suggesting that the proliferation of cells in the state.Conclusions ADMA can promote the viability and the proliferation of monocytes,and contribute to the formation of atherosclerosis.