抑制素A及促性腺激素对小鼠未成熟卵母细胞体外成熟的影响

The effect of inhibin A and gonadotrophic hormone on the in vitro maturation of mice immature oocytes

目的:探讨抑制素A(InhibinA,INHA)对小鼠未成熟卵母细胞体外成熟(In vitro maturation,IVM)的影响。方法:从孕马血清促性腺激素(Pregnant mare serum gonadotrophin,PMSG)组(使用PMSG组,16例)及无PMSG组(未使用PMSG组,20例)获得的生殖泡期(Germinal vescle,GV)卵母细胞在不同浓度INH A的培养液中进行体外培养,观察第一极体(The first polar body,PB1)排出和皮质颗粒(Cortical granules,CG)染色情况。结果:PMSG组获卵数为(17.2±2.6)个/只,无PMSG组获卵数为(15.9±1.8)个/只(P>0.05);PMSG组与无PMSG组的PB1率分别为57.7%、50.7%(P>0.05)。PMSG组中INH A200、350ng/ml组的PB1率较对照组高(P<0.05);无PMSG组中各INH A组PB1率与对照组比较无显著差异(P>0.05);结论:INH A和PMSG对体外培养GV期卵母细胞核成熟有促进作用。

Objective：To investigate the significance of inhibin A（INH A）in the in vitromaturation.Methods：All oocytes extracted were divided into PMSG group（mice＇s peritoneal cavity was injected with PMSG（pregnant mare serum gonadotrophin）before getting oocytes） and none PMSG group.The germinal vesicle（GV）oocytes isolated from rats were cultured in the INH A solutions at concentrations of 0（control）,50,100,200 and 350 ng/ml IVF plus culture media.Extrusion of the first polar body（PB1）and staining of cortical granules（CG）were observed.Results：We can go（t17.2±2.6）oocytes/mice with using PMSG,and we can got（15.9±1.8）oocytes/mice without PMSG（P〉0.05）.There was no difference in the rate of PB1 between PMSG group and none PMSG group.In PMSG group,INH A solutions at concentrations of 200 ng/ml,350 ng/ml had higer rate of PB1 than that in control group（P〈0.05）;In the none PMSG group,each INH A group had no significant difference in the rate of PB1.Conclusion：INH A and PMSG maybe played an important role in oocytes maturation from GV phase in vitro.