大蒜素及缺血预处理对大鼠肾缺血再灌注损伤的影响及机制研究

Effect of allitride injection and ischemic preconditioning on renal ischemia/reperfusion injury in rats

目的:探讨缺血预处理和大蒜素注射液预处理对大鼠肾缺血再灌注损伤的影响及机制。方法:建立大鼠肾缺血再灌注损伤模型。将75只SD大鼠随机分成对照组(Control group,CON组)、缺血再灌注组(Ischemia reperfusion,IR组)、缺血预处理组(Ischemic preconditioning group,IPC组)、大蒜素预处理组(Allitride preconditioning group,APC组)、大蒜素及缺血预处理(Allitridpreconditi oning and ischemic preconditioning group,AIPC组)共5组,每组15只。各组均于术后24h采集肾组织及血样标本,用HE法观察肾组织病理形态、测定血尿素氮(Blood urea nitrogen,BUN)、肌酐(Creatinine,Cr)含量、TBA法测定丙二醛(Malonaldehyde,MDA)含量、黄嘌呤氧化酶法测定超氧化物歧化酶(Superoxide dismutase,SOD)活性;免疫组化法观察肾组织中粘附分子(Interce llular adhesionmolecule-1,ICAM-1)的表达。结果:IPC或APC后HE染色坏死较IR组减少,BUN、Cr、MDA含量较IR组下降(P<0.01),SOD活性较IR组升高(P<0.01),肾组织ICAM-1表达较IR组下降(P<0.01)。AIPC处理后各项指标较IPC或APC单一处理有显著区别(P<0.01)。结论:缺血预处理和大蒜素预处理对肾缺血再灌注有保护作用,两种处理因素对大鼠肾缺血再灌注损伤的保护有协同作用,可能是通过下调ICAM-1的表达实现的。

Objective：To investigate the effect of allitride injection preconditioning and ischemic preconditioning on renal ischemia/ reperfusion（I/R）injury in rats.Methods：Rat＇s I/R injury model was established.A total of 75 rats were randomized into five groups（n=15,in each）;control group（CON）,I/R control group（IR）,ischemic preconditioninggroup（IPC）,allitride preconditioninggroup（APC）,allitride preconditioning and ischemic preconditioning group（AIPC）.Kidney tissue and blood specimen were collected from all groups 24 hours after operation.The changes of the renal pathological morphology were observed by hematoxylin-eosine（HE）staining.The levels of serum creatinine（Cr）and urea nitrogen（BUN） were determined.The levels of malonaldehyde（MDA）were determined by thibabituric acid（TBA）.The levels of superoxide dismutase（SOD）were determined by xanthine oxidase.Intercellular adhesion molecule-1（ICAM-1）expression in kidney was tested by immunohistoehemistry.Results：IPC or APC group had less necrosis in HE staining than IR group.BUN,Cr,MDA contents in IPC or APC group are less than that in IR group（P〈0.01）.SOD activity in IPC or APC group is higher than IR group（P〈0.01）.ICAM-1 expression in kidney in IPC or APC group is lower than IR group（P〈0.01）.After AIPC process,all indicators substantially differed from those only after IPC or APC（P〈0.01）.Conclusion：Ischemic preconditioning or allitride preconditioning would protect kidney IR injury,and that the two factor would have synergy in protecting kidney IR injury,probably by down regulating ICAM-1 expression.