口腔颊粘膜癌组织中CD1a^+的树突状细胞的免疫组化分析

Immunohistochemical analysis of expression of CD1a of dendritic cell in oral squamous cell carcinoma of buccal mucosa

目的通过免疫组化技术分析树突状细胞(dendritic cell,DC)在口腔颊粘膜鳞状细胞癌(oralsquamous cell carcinoma,OSCC)中的表达,探讨其在肿瘤组织中的功能状态。方法随机选取22例临床收集的未经任何其他非手术治疗的颊粘膜OSCC患者标本,以10例正常口腔粘膜(normal mucosa,NM)和10例口腔非特异性炎症上皮(inflammatory mucosa,IM)为对照,采用CD1a单抗通过免疫组化技术标记上皮组织中的DCs,结果进行统计学分析。结果 NM组中有3例未见CD1a+DC表达,OSCC组及IM组中各有1例未见CD1a+DC表达,OSCC组与NM组、OSCC组与IM组之间CD1a+DC的计数均有显著性差异(P<0.05);肿瘤不同病理分级中,癌旁组织(ESCC)中CD1a+DC的数量在OSCCⅠ级与Ⅱ级间有显著性差异(P<0.05);ESCC中CD1a+DC在有或无淋巴结转移中的差别有显著意义(P<0.05)。结论 ESCC中CD1a+DC与肿瘤分级呈显著负相关,与颊癌的预后相关。

Objective To evaluate the functional status of dendritic cells （DCs） in oral squamous cell carcinoma （OSCC） of buccal mucosa by analyzing expression of CD1a.Methods Twenty-two specimens of OSCC from buccal mucosa treated with surgery were randomly selected as group OSCC 10,with respectively specimens of normal mucosa （NM） and 10 specimens of inflammatory mucosa （IM） from buccal mucosa were chosen as the controls. The pathologic specimens were immunostained against CD1a monoclonal antibody by avidin-biotin peroxidase complex （ABC） technique.Results Negative expression of CD1a DCs were observed respectively in 3 specimens of NM,1 specimen of IM and 1 specimen of OSCC. A statistical significance existed between group NM and group OSCC,and the same case to group IM and group OSCC. The number of CD1a-positive DCs in epithelium adjacent to carcinoma （EAC） existed a statistical significance between stage Ⅰ and stage Ⅱ of OSCC.Conclusion The results suggest that the grade of OSCC is negatively related to the number of CD1a-positive DCs in EAC and is associated with improved survival in patients with OSCC.