摘要
目的:观察Ⅲ类组蛋白去乙酰基酶1(Sirt1)在异丙肾上腺素(ISO)诱导的小鼠心肌肥大中的表达及作用。方法:昆明小鼠随机分为正常对照组、ISO模型组和ISO+NAM(烟酰胺,Sirt1抑制剂)组。皮下注射ISO诱导小鼠心肌肥大。以心脏重量指数(心脏重量/体重)、HE染色、透射电镜以及脑钠肽(BNP)mRNA表达水平观察心肌肥大的变化。同时采用RT-PCR、Western blotting检测各组心肌Sirt1 mRNA、蛋白质的表达。结果:与对照组相比,心脏重量指数,HE染色,透射电镜以及BNP mRNA表达水平均显示皮下注射ISO可明显诱导小鼠心肌肥大;Sirt1在心肌肥大模型组表达显著增多(P<0.01),其抑制剂NAM可明显减轻ISO诱导的心肌肥大(P<0.05),并降低Sirt1的表达(P<0.01)。结论:Sirt1的激活可能参与ISO诱导的小鼠心肌肥大。
AIM：To investigate the expression and function of sirtuin 1 （Sirt1）,one of the class III histone deacetylases,in hypertrophied mouse myocardium induced by isorenin （ISO）. METHODS：The Kunming mice were randomly divided into 3 groups：control group,ISO group and ISO ＋ nicotinamide （NAM） group. The myocardial hypertrophy was induced by dorsal subcutaneous injection of isorenin. Nicotinamide,an inhibitor against Sirt1,was given by peritoneal injection. Heart weight index （heart weight/body weight）,hematoxylin and eosin staining,transmission electron microscope and mRNA expression of brain natriuretic peptide （BNP） were observed to identify the myocardial hypertrophy. The expression of Sirt1 in mRNA and protein levels was detected by RTPCR and Western blotting,respectively. RESULTS：Compared to control group,the results of heart weight index,hematoxylin and eosin staining,the observation of transmission electron microscopy and mRNA expression of BNP showed that the mouse myocardial hypertrophy was induced by isorenin successfully. The Sirt1 expression was increased in hypertrophy model group （P 0. 01 vs control group）. Treatment with nicotinamide inhibited the cardiac hypertrophy induced by ISO （P 0. 05 vs ISO group） and decreased the expression of Sirt1 （P 0. 01 vs ISO group）. CONCLUSION：Activation of Sirt1 might be involved in the process of myocardial hypertrophy stimulated by isorenin in mice.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第5期844-847,共4页
Chinese Journal of Pathophysiology
基金
黑龙江省教育厅科学技术研究资助项目(No.11521078)
哈医大基础学科青年科学基金资助项目(No.060016)
