期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

3-氧十二烷酰高丝氨酸内酯对淋巴细胞Toll样受体2和4的影响

Expression of Toll-like receptor 2 and 4 in lymphocytes stimulated with N-(3-oxododecanoyl)-L-homo-serine lactone
下载PDF
导出
摘要 目的:研究铜绿假单胞菌群体感应(QS)系统信号分子3-氧十二烷酰高丝氨酸内酯(3-O-C12-HSL)对人外周血淋巴细胞增殖、TLR2/4mRNA表达以及单个核细胞分泌TNF-α的影响。方法:自健康献血者血液中分离单个核细胞进行体外培养,并以0、1、10、50、100μmol/L3-O-C12-HSL作用12h,分离淋巴细胞,采用MTT法,观察不同浓度的3-O-C12-HSL分别作用对人淋巴细胞生长增殖的影响。用RT-PCR测定淋巴细胞TLR2/4mRNA表达。收集上清,以双抗体夹心酶联免疫吸附试验(ELISA)法测TNF-α。结果:3-O-C12-HSL刺激12h后淋巴细胞TLR2/4mRNA表达增高,且呈剂量依赖关系,TLR2/4mRNA在100μmol/L时最为显著(0.723±0.005,0.363±0.013),P<0.05。TLR2变化较TLR4明显,P<0.05。3-O-C12-HSL对淋巴细胞增殖有促进作用,呈剂量依赖关系,在100μmol/L时最为显著(0.463±0.052),P<0.05。3-O-C12-HSL能抑制单个核细胞分泌TNF-α,且呈剂量依赖关系,在100μmol/L时最为显著(267.090±13.878),P<0.05。结论:3-O-C12-HSL可以激发淋巴细胞细胞增殖,上调Toll样受体的表达水平,Toll样受体表达上调抑制TNF-α表达,获得性免疫对固有免疫有反馈作用。
作者 芦起 余加林 杨锡强 刘伟
机构地区 重庆医科大学附属儿童医院
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2010年第5期1003-1005,共3页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目(No.30772363 No.30901279) 重庆市自然科学基金资助项目(No.2007BB5282) 重庆医科大学附属儿童医院院级项目
关键词 假单胞菌 铜绿 受体 淋巴细胞 3-氧十二烷酰高丝氨酸内酯 Pseudomonas aeruginosa Receptors lymphocyte N-3-oxododecanoyl-L-homoserine lactone
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献11

  • 1Bauer TT,Torres A,Ferrer R,et al.Biofilm formation in endotracheal tubes.Association between pneumonia and the persistence of pathogens[J].Monaldi Arch Chest Dis,2002,57(1):84-87.
  • 2Saleh AC,Figarella W,Kammouni S,et al.Pseudomonas aeruginosa quorum-sensing signal molecule N-(3-oxododecanoyl)-L-homoserine lactone inhibits expression of P2Y receptors in cystic fibrosis tracheal gland cells[J].Infect Immun,1999,67(10):5076-5082.
  • 3Smith RS,Harris SG,Phipps R,et al.The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl) homoserine lactone contributes to virulence and induces inflammation in vivo[J].J Bacteriol,2002,184(4):1132-1139.
  • 4Zhu H,Conibear TC,Thuruthyil SJ,et al.Pseudomonas aeruginosa quorum-sensing signal molecules induce IL-8 production by human corneal epithelial cells[J].Eye Contact Lens,2008,34(3):179-181.
  • 5Tateda K,Ishii Y,Horikawa M,et al.The Pseudomonas aeruginosa autoinducer N-3-oxododecanoyl homoserine lactone accelerates apoptosis in macrophages and neutrophils[J].Infect Immun,2003,71(10):5785-5793.
  • 6田青,周恒,王蕾,甘文云,郭海鹏,姜明,邓伟,艾文,卞洲艳,严玲,沈涤非,唐其柱.Toll样受体3在自身免疫性心肌炎小鼠心肌组织中的表达及意义[J].中国病理生理杂志,2009,25(12):2323-2328. 被引量：2
  • 7刘颖,郑春泉.Toll样受体和免疫性疾病[J].中国眼耳鼻喉科杂志,2008,8(1):58-60. 被引量：3
  • 8Sutmuller RP,den Brok MH,Kramer M,et a1.Toll-like receptor 2 controls expansion and function of regulatory T cells[J].J Clin Invest,2006,116(2):485-494.
  • 9Caramalh I,Lopes-carvalho T,Ostler D,et al.Regulatory T cells selectively express Toll-like receptors and activated by lipopolysaccharide[J].J Exp Med,2003,197(4):403-411.
  • 10Hooi DS,Bycroft BW,Chhabra SR,et al.Differential immune modulatory activity of Pseudomonas aeruginosa quorum-sensing signal molecules[J].Infect Immun,2004,72(11):6463-6470.

二级参考文献40

  • 1Eriksson U, Penninger JM. Autoimmune heart failure : new understandings of pathogenesis [ J ]. Int J Biochem Cell Biol, 2005, 37(1) :27 -32.
  • 2Klingel K, Hohenadl C, Canu A, et al. Ongoing enterovirus - induced myocarditis is associated with persistent heart muscle infection: quantitative analysis of virus replication, tissue damage, and inflammation [ J]. Proc Natl Acad Sci USA, 1992, 89( 1 ) :314 - 318.
  • 3Alexopoulou L, Holt AC, Medzhitov R, et al. Recognition of double - stranded RNA and activation of NF - kappa B by Toll - like receptor 3 [ J ]. Nature, 2001, 413 (6857) : 732 - 738.
  • 4Eriksson U, Ricci R, Hunziker L, et al. Dendritic cell - induced autoimmune heart failure requires cooperation between adaptive and innate immunity[J]. Nat Med, 2003, 9(12) :1484 - 1490.
  • 5Kawai T, Akira S. TLR signaling [ J ]. Semin Immunol, 2007, 19(1) :24 -32.
  • 6Janssens S, Beyaert R. Role of Toll - like receptors in pathogen recognition[ J]. Clin Microbiol Rev, 2003, 16 (4) :637 - 646.
  • 7Shen J, O'Brien D, Xu Y. Matrix metalloproteinase -2 contributes to tumor necrosis factor alpha induced apoptosis in cultured rat cardiac myocytes [ J ]. Biochem Biophys Res Commun, 2006, 347 (4) : 1011 - 1020.
  • 8Yan L, Tang Q, Shen D, et al. SOCS - 1 inhibits TNF - α - induced Cardiomyocyte apoptosis via ERK1/2 pathway activation [ J ]. Inflammation, 2008, 31 ( 3 ) : 180 - 188.
  • 9NOGUCHI E, NISHIMURA F, FUKAI H, et al. An association study of asthma and total serum immunoglobin E levels for Toll-like receptor polymorphisms in a Japanese population [ J ]. Clin Exp Allergy,2004,34(2) :177-183.
  • 10BACHAR O, ADNER M, UDDMAN R, et al. Toll-like receptor stimulation induces airway hyper-responsiveness to bradykinin, an effect mediated by JNK and NF-kappa B signaling pathways[J]. Eur J Immunol,2004,34(4 ) :1196-1207.

共引文献3

中国病理生理杂志
2010年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部