脂联素对高糖中hRPE细胞活性及T-cad表达的影响

Effects of Adiponectin on Cell Activity and Expression of T-cadherin in Human RPE Cells Cultured with High D-glucose

目的观察脂联素对高糖环境下人视网膜色素上皮(hRPE)细胞活性及T-钙黏蛋白(T-cad)表达的影响,以阐明脂联素和T-cad对糖尿病视网膜病变(DR)的影响。方法①将hRPE细胞随机分为正常对照组(5.5mmol/L葡萄糖)、高糖组(33mmol/L葡萄糖)、脂联素组(33mmol/L+2.5μg/ml脂联素),运用MTT法测定不同情况下48h后对细胞活性的影响;②再将上述各组细胞分别培养24h、48h、72h后,运用荧光定量PCR测定T-cad的表达水平。结果①高糖组细胞活性降低,加入脂联素后细胞活力比高糖组升高;②随时间的延长,高糖组T-cad表达下降,脂联素组T-cad表达增加。结论脂联素可通过Tcad保护hRPE细胞的功能。

Objective To investigate the effects of adiponectin on cell activity and expression of T - cadherin in human retinal pigment epithelial（hRPE） cells cultured with high glucose. Methods ①The hRPE cells were divided into three groups： normal glucose group（5.5mmol/L glucose） , high glucose group （33mmol/L glucose） , high glucose plus adiponectin group （ 33mmol/L glucose ＋ 2.5ug/ ml adiponectin）. 48h later, the cell activity was observed by MTT assay. ②After the above groups were cultured respectively for 24h ,48h, 72h again, the expression of T - cadherin mRNA was observed by RT - PCR. Results ①Cell activity in high glucose group significantly decreased. Cell activity adiponectin group was higher than that in the high glucose group.②With the extension of time , the expression of T- cadherin decreased in high glucose group, but increased in adiponectin group. Conclusion Adiponectin may be protect the hRPE cells by T - cadherin.