家兔非酒精性脂肪肝血生化指标研究

Study on Blood Biochemical Indexes of NAFLD in Rabbits

目的建立不同程度家兔非酒精性脂肪肝模型,观察非酒精性脂肪肝程度与血生化指标的关系。方法选用32只新西兰家兔,随机分为对照组(n=8)和模型组3组(每组n=8),采用高脂高糖饲料建立不同程度脂肪肝模型,分别于4、6、8周检测家兔血清TG、TC、AST、ALT,并进行病理学检查。结果高脂高糖饲料喂养4周后可形成轻度的脂肪肝并出现血脂的升高,随着脂肪肝病变程度的发展,血清TG水平持续升高。结论血生化指标对脂肪肝有一定的诊断价值。

Objective To determine the relationship between blood biochemical indexes and severity of NAFLD by establishing various models of NAFLD with different degrees in rabbits. Methods Thirty - two healthy rabbits were randomly divided into normal control （ n = 8 ） and three experimental groups （ n = 24）. Fatty liver animal models were established by giving high fat, high sugar diet. The serum TG,TC,AST,ALT and pathological evaluation were detected respectively after 4, 6 and 8 weeks. Results The rabbits developed hyper-lipidemia and slight fatty liver after four weeks. Along with the worsening of fat liver, the serum TG level kept rising. Conclusion The blood biochemical indexes can serve as a reliable technique for diagnosing fatty liver disease in clinical.