重庆地区表观健康猪中猪链球菌的检测

Detection of the Streptococcus suis of Clinical Healthy Pigs in Chongqing

本研究旨在调查重庆地区表观健康猪的猪链球菌带菌情况,并揭示健康猪群携带猪链球菌的公共卫生学意义。随机采集重庆市17个区县(市)定点屠宰场表观健康猪的腭扁桃体1 360份,进行猪链球菌的分离鉴定,并对致病性较强的1、2、7、9、14型及1/2型进行分型与毒力因子分析。结果共分离到225株猪链球菌,分离率为16.54%;检出猪链球菌2型4株,猪链球菌7型3株,猪链球菌9型3株,猪链球菌1/2型1株;毒力因子谱分析发现多数(10/11)分离株缺失部分毒力因子,但也存在具有全部已知毒力因子的强毒株,且在国内首次检出小片段mrp型猪链球菌1/2型1株和7型2株。结果提示,重庆地区健康猪群带菌现象普遍,且流行菌株具有与国外不同的特点,对屠宰场工作人员健康造成威胁。

This research was conducted to investigate the Streptococcus suis carrier state of pigs in Chongqing and reveal the importance of the state in public health.One thousand three hundred and sixty palatine tonsils of clinical healthy slaughter pigs were collected from 17 abattoirs in different district of Chongqing.Streptococcus suis was isolated from the samples.The serotype 1,2,7,9,14 and 1/2 was identified,the virulence-associated genes profile and multilocus sequence typing scheme of which was analyzed.Two hundred and twenty-five strains of Streptococcus suis were isolated from the samples,and the isolation rate was 16.54%.Of the 225 isolates recovered from slaughter pigs,4 Streptococcus suis type 2 isolates,3 Streptococcus suis type 7 isolates,3 Streptococcus suis type 9 isolates were identified and 1 Streptococcus suis type 1/2 isolate was detected.Virulence-associated gene profiling analysis showed that majority isolates（10/11） were defective of some virulence-associated gene;but the isolates possessing all known virulence-associated gene also existed;the short type mrp phenotype Streptococcus suis type 1/2（1 isolate） and type 7（2 isolates） isolates were firstly identified in China.It is obvious that the carrier state of clinical healthy pigs bring potential infection jeopardize to butcher;the prevalent isolates are little different from the other country.