p53基因codon72多态性与乳腺癌的相关性研究

Study on p53 Codon 72 Polymorphism in 277 Patients with Breast Cancer

目的：研究p53基因codon72多态性与乳腺癌患者的年龄、病理分期、淋巴结转移、雌激素受体（ER）、孕激素受体（PR）、c—erbB-2、P53蛋白表达情况的相关性。方法：TaqMan探针方法检测277例乳腺癌患者血液标本的p53基因codon72多态性。免疫组化SP法检测匹配肿瘤纽织中ER、PR、c-erbB-2和P53蛋白的表达情况。SPSS16．0软件行统计学分析，p53基因多态性与病理学特征关系用x^2检验，非条件Logistic回归分析基因多态性与ER、PR、c—erbB-2、P53蛋白表达的相关性，计算OR值及其95％可信区间（95％CI）。P〈0．05为差异有统计学意义。结果：p53基因codon72基因型为CC／CG／GG．频率分别为22．0％、513％和26．7％，携带CC、CG、GG基因型的患者发病年龄逐渐降低，但无统计学差异；p53基因codon72多态性与临床病理学特征无关，与ER、PR、c—erbB-2和P53蛋白表达无相关性（P〉0．05）。肿瘤组织P53蛋白表达与ER、PR、c—erbB-2蛋白表达密切相关（x^2=15．492，P=0．000；x^2=3．970，P=0．046；x^2=17．956，P=0．000）。结论：p53基因codon72多态性与P55蛋白表达及病理学特征无相关性，P53蛋白表达与ER、PR、C—erbB-2蛋白表达关系密切。p53基因codon72基因型与患者发病年龄的关系有待扩大样本量进一步研究。

Objective： To investigate the correlation of p53 codon 72 polymorphism with clinicopathologic features of breast cancer patients including age, tumor staging, lymph node status, receptor status of estrogen and progestin, expression of P53 protein and c-erbB-2. Methods： Blood samples were collected from 277 women with primary breast cancer. TaqMan was used to determine the genotypes of p53 codon 72 polymorphism. Expression of ER, PR, c-erbB-2 and P53 proteins were detected with immunohistochemistry. SPSS 16.0 software was employed for statistical analysis and the relationship of p53 polymorphism with all of the clinicopathologic parameters was evaluated with x^2 test. Furthermore, the association of p53 polymorphism with ER, PR, c-erbB-2 and P53 protein expression was analyzed by logistic regression analysis. Results： The frequency of CC, CG, and GG at p53 codon 72 was 22.0%, 51.3%, and 26.7%, respectively. No correlation was found between genotype distribution and clinicopathologic parameters of disease outcome. There was no correlation between p53 condon 72 polymorphisms and the expression of ER, PR, c-erbB-2, or P53 proteins （P〉0.05）. P53 protein expression was associated with expression of ER, PR, and c-erbB-2 proteins （x^2=13.492, P=-0.000; x^2=3.970, P=0.046; x^2= 17.956, P=0.000）. Conclusion： There is no correlation between p53 codon 72 polymorphism and clinicopathologic parameters of breast cancer. P53 protein is significantly associated with ER, PR and c-erbB-2 expression. Further study using a larger sample is warranted.