活血补肾汤对兔激素性股骨头坏死VEGF的影响

The Effect of Huoxue Bushen Decoction on Expression of VEGF in Steroid-induced Osteonecrosis of the Femoral Head of Rabbit

目的:探讨激素性股骨头坏死(SANFH)过程中血管内皮生长因子(VEGF)在股骨头局部的表达改变及活血补肾汤对VEGF表达的影响。方法:130只新西兰大白兔随机均分为正常对照组、激素造模组、高、中、低剂量中药治疗组,造模成功后,各组于给药后的4、8、12周分别处死后取一侧股骨头,免疫组化SP法测定VEGF含量,并行常规病理组织学检查。结果:第4周时,高、中、低剂量组及模型组明显低于正常对照组(P<0.01)。即表达增强。第8周:高、中、低剂量中药组VEGF灰度值比模型组均显著降低(P<0.01),即VEGF表达明显增强;12周时高、中、低剂量组的VEGF灰度表达值明显低于模型组(P<0.01),即VEGF表达明显强于模型组。结论:激素可导致股骨头局部VEGF的表达明显降低(4周时有代偿性增高),活血补肾汤可显著提高VEGF的表达,促进血管及新骨生成。可能是其治疗激素性股骨头坏死的主要作用机制之一。

Objective：To study the expression changes of VEGF in the femoral head during steroid induced avascular necrosis of femoral head（SANFH）and the influence of Huoxue Bushen Decoction（HBD）on VEGF expression.Methods：One hundred and thirty rabbits were divided into control group,model group and HBD treated groups（high,medium and low dosage groups）.On the time of 4,8,and 12 weeks after treatment,rabbit＇s femoral head from each group was taken for histological observation and detection of VEGF using immunohistochemistry staining.Results：On the 4th week,the grey scale of VEGF in the model group and HBD groups was significantly lower than in control group（P0.01）.Since the 8th week,grey scale of VEGF in HBD groups was obviously lower than that in model group（P0.01）.The same trend was found on the 12th week.Conclusion：Steroid can suppress the expression of VEGF in femoral head,while HBD can reverse the process,which may be one of the mechanisms of HBD in treating SANFH.