RANTES-403G/A、MCP-1-2518A/G基因多态性与呼吸道合胞病毒毛细支气管炎易感性的相关性

Correlation of cheomkine gene polymorphisms with the susceptibility of respiratory syncytial virus bronchiolitis

目的:探讨调节激活正常T细胞表达、分泌的细胞因子(RANTES)-403G/A、单核细胞趋化蛋白1(MCP-1)-2518A/G基因多态性与呼吸道合胞病毒(RSV)毛细支气管炎易感性的相关性。方法:应用聚合酶链反应-限制性酶切片段长度多态性技术(PCR-RFLP)检测150例RSV毛细支气管炎患儿和120例正常儿童上述位点单核苷酸多态性(SNPs)。结果:病例组RANTES-403G/A基因型和等位基因型频率分别为GG20.7%、GA63.3%、AA16.0%、G52.3%、A47.7%,对照组为GG37.5%、GA46.7%、AA15.8%、G60.8%、A39.2%,两组基因型频率差异有显著性(P<0.05);病例组A等位基因频率高于对照组(P<0.05),与G等位基因携带者相比,A等位基因携带者患病风险增加(OR=1.415,P<0.05)。两组MCP-1-2518基因型和等位基因频率差异无显著性(均P>0.05)。结论:RANTES-403A等位基因与RSV毛细支气管炎易感性相关,其可能是影响发病的一个重要候选基因;未发现MCP-1-2518A/G基因多态性与RSV毛细支气管炎易感性存在关联。

Objective： To explore the correlation of regulated on activation,normal T cell expressed and secreted （RANTES） and monocyte chemotactic protein 1（MCP-1） gene polymorphisms with the susceptibility of respiratory syncytial virus （RSV） bronchiolitis.Methods： RANTES403G/A and MCP-1-2518A/G were genotyped with polymerase chain reaction-restriction fragment length polymorphism in 150 children with RSV bronchiolitis and 120 healthy subjects.Results： The frequency of each genotype for RANTES-403G/A was 20.7% （GG）,63.3% （GA）,16.0% （AA） in case group and 37.5% （GG）,46.7% （GA）,15.8% （AA） in control one,respectively.There was significant difference between two groups （P 0.05）.The frequency of each allele was 52.3% （G）,47.7% （A） in case group,and 60.8%（G）,39.2% （A） in control group.The frequency of A allele was significantly increased in case group （P 0.05）;Children carrying with A allele increased risk for RSV bronchiolitis compared with those with G allele （OR=1.415,P 0.05）.There were no significant differences for genotype and allele frequency of MCP-1-2518A/G between two groups （P 0.05）.Conclusion： RANTES-403A allele is associated with the susceptibility and may be one of candidate genes for RSV bronchiolitis.No correlation is found between MCP1-2518A/G gene polymorphisms and the susceptibility of RSV bronchiolitis.