严重急性呼吸系统综合征冠状病毒N蛋白对甘油三酯和总胆固醇含量的影响

Effects of Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus on Blood Triglyceride and Cholesterol

目的:研究严重急性呼吸系统综合征冠状病毒(SARS-CoV)N蛋白对甘油三酯和总胆固醇含量的影响。方法:检测分析144例SARS患者甘油三脂和总胆固醇含量在发病后的变化;将小鼠随机分组,分别注射生理盐水和SARS-CoV N蛋白,连续给药9 d后检测小鼠血清中甘油三酯和总胆固醇含量的变化。结果:SARS患者的甘油三酯和总胆固醇含量随发病时间有升高变化(P<0.05),在发病40 d前后甘油三酯和总胆固醇含量超标的病例数占本时间段内的病例数的比例显著高于其他时间段,而且超标的含量也明显升高。SARS-CoV N蛋白使小鼠体重明显高于对照组(P<0.05),甘油三酯含量在39和51 d明显高于对照组(P<0.05),总胆固醇含量在21和39 d也明显高于对照组(P<0.05)。结论:SARS-CoV N蛋白可以升高小鼠的甘油三酯和总胆固醇的含量,其升高最为明显的时间段与SARS患者的甘油三酯和总胆固醇含量升高的时间段基本一致。由此推测,SARS-CoV N蛋白可能是促使SARS患者发病后甘油三酯和总胆固醇含量升高的重要原因。

Objective：To investigate the effects of nucleocapsid protein（N protein） of severe acute respiratory syndrome coronavirus（SARS-CoV） on blood triglyceride and cholesterol.Methods：144 cases of SARS patients were analysed by testing the concentration of their blood triglyceride and cholesterol.Simultaneously twenty mice were randomly assigned to two groups which were continuously given physiological saline and SARS-CoV N protein for 9 days,then the changes of body weight,blood triglyceride and cholesterol were tested.Results：The concentration of blood triglyceride and cholesterol in the bodies of SARS patients increases in line with disease time（P0.05）.The rising of blood triglyceride and cholesterol of patients around 40th day were observed,and the proportion of the patients who has high concentration of triglyceride or cholesterol around 40th day is higher than the proportion in other period.The body weight of mice in SARS-CoV N protein group was significantly higher than those in control group（P0.05）.The concentration of triglyceride of mice in SARS-CoV N protein group was obviously higher than those in control group in the 39th and 51th day.The concentration of cholesterol in SARS-CoV N protein group was obviously higher than those in control group in the 21th and the 39th day（P0.05）.Conclusion：Triglyc-eride and cholesterol levels of SARS patients changed obviously through disease time,and the levels obviously rose around 40 days.SARS-CoV N protein can increase the body weight and the levels of triglyceride and cholesterol of mice,and the increment was obvious around the 39th day which is consistent with SARS patients.It can be seen that SARS-CoV N protein is the important cause of increasing the concentration of triglyceride and cholesterol after SARS infection.