原因不明月经过少子宫内膜雌激素受体β基因多态性及其表达

Study on the Estrogen Receptor β Gene Polymorphism and its Expression in Unknown Aetiology Hypomenorrhea

目的:探讨原因不明月经过少子宫内膜雌激素受体β(ERβ)基因多态性及其与表达的关系。方法:从2组人群中分别选取原因不明月经过少子宫内膜组织40例、月经量正常子宫内膜组织40例,通过逆转录-多聚酶链反应(RT-PCR)和Western印迹检测ERβ的表达;分析实验组与对照组ERβmRNA及蛋白质的表达情况,将ERβ基因的各基因型、等位基因型与子宫内膜ERβmRNA及蛋白质的表达之间分别进行比较。结果:实验组ERβmRNA表达量为0.6457±0.2957,对照组为0.9637±0.3621,实验组比对照组表达下调,差异有统计学意义(t=-6.589,P<0.001);实验组ERβ蛋白质表达量为347.37±30.35,对照组为445.21±45.67,实验组比对照组表达下调,差异有统计学意义(t=-4.353,P<0.001);2组RsaⅠ基因型、AluⅠ基因型、CA重复序列基因型ERβ表达差异均无统计学意义。结论:原因不明月经过少患者ERβmRNA及蛋白质在子宫内膜中的表达低于月经量正常子宫内膜,可能与月经量减少有关;2组人群ERβ基因RsaⅠ、AluⅠ、CA重复序列基因型及等位基因型子宫内膜表达无差异。

Objective：To study the relationship between estrogen receptor β（ERβ） gene polymorphism and its ex-pression in unknown aetiological hypomenorrhea.Methods：The tissues of 40 unknown aetiological hypomenorrhea specimens and 40 normal menstrual volume endometria were chosen.The RT-PCR and Western blot were used to investigate the expression of the ERβ at mRNA and protein level.The mRNA and protein expression were com-pared with genotype and allelotype respectively.Results：The ERβ mRNA expression amount was 0.6457±0.2957 in case group and 0.9637±0.3621 in control group.The mRNA expression of ERβ markedly decreased in unknown aetiological hypomenorrhea compared with normal menstrual volume endometria,the difference was statistical signifi-cant（t=-6.589,P0.001）.The ERβ protein expression amount was 347.37±30.35 in case group and 445.21±45.67 in control group,the difference was statistical significant（t=-4.353,P0.001）.ERβ expression differences were not sta-tistically significant among RsaⅠ genotype,AluⅠ genotype,CA repeat genotype.Conclusion：The mRNA and pro-tein expression of ERβ decreases in unknown aetiological hypomenorrhea compared with normal menstrual volume endometria,which may be related to the menstrual volume of unknown aetiological hypomenorrhea.The expression of ERβ is not related to RsaⅠ、AluⅠ and CA dinucleotide repeat sequence genotypes and allelotypes.