不同饲养层对鸡胚胎干细胞培养效果的影响

Effects of Different Feeders on Cultured Chicken Embryonic Stem Cells

目的:为探索鸡胚胎干细胞培养的优化条件,比较不同饲养层对鸡胚胎干细胞离体培养的效果。方法:用传至第2代的鸡胚成纤维细胞与鸭胚成纤维细胞,经丝裂霉素处理后制作饲养层,比较这2种饲养层以及不用饲养层对鸡胚胎干细胞离体培养效果的影响。结果:在以鸡胚成纤维细胞和鸭胚成纤维细胞作为饲养层的培养体系中,鸡胚胎干细胞均可保持良好的生长状态,而且2种饲养层对鸡胚胎干细胞克隆形成的影响差异不显著(P>0.05)。结论:鸡胚成纤维细胞和鸭胚成纤维细胞均可作为较好的饲养层细胞用于鸡胚胎干细胞的离体培养。

Objective：To research the optimal culture condition of chicken embryonic stem cells,compare the effect of different feeders on cultured chicken embryonic stem cells in vitro.Methods：The second generation chicken embryonic fibroblast（CEF） and duck embryonic fibroblast（DEF） were used to make feeders after treated with mitomycin C.The state of chicken embryonic stem cells were observed and the number of cell clones was counted on the culture system with those two feeder layers or feeder layer free.Results：The results showed that chicken embryonic stem cells growth well both on the CEF and DEF feeder,and the number of cell clones was no distinct difference between CEF and DEF（P0.05）.Conclusion：The results indicated that both of CEF and DEF can be used as feeders to culture chicken embryonic stem cells.