电磁场防治去卵巢小鼠骨质疏松的实验研究

The effects of electromagnetic fields on osteoporosis in ovariectomized mice

目的 评价电磁场防治去卵巢小鼠骨质疏松的效果及作用机制.方法 将60只雌性小鼠分为正常组（A组）、模型组（B组）、电磁场防治组（C组）及尼尔雌醇防治组（D组）.除A组外其余各组均切除双侧卵巢,C组小鼠接受15 Hz、1.0 mT电磁场刺激,D组小鼠接受尼尔雌醇1.5 ms/kg体重灌胃,1次/周.实验结束前检测腰椎骨密度.分别于术后第4,8,12周进行内眦静脉丛取血,采用碱性磷酸酶（ALP）检测试剂盒和小鼠骨钙蛋白（BGP）酶联免疫吸附反应（ELISA）检测试剂盒检测血清ALP活性及BGP含量,并检测血清钙和雌二醇（E_2）含量,于术后第6,12周末行腰椎椎体病理学切片检查,观察并分析骨小梁形态及数量的变化.结果 与B组相比,C组血清中ALP活性明显提高（P〈0.05）,血清中BGP和血清钙含量增加（P〈0.05）,腰椎椎体骨小梁的吸收减少.结论 15 Hz、1.0 mT的电磁场对去卵巢小鼠骨质疏松有显著的防治作用.

Objective To study the effects of electromagnetic field （EMF） exposure on osteoporosis in ovariectomized mice. Methods Sixty 8-week-old female Kunming mice were divided into four groups at random： a sham operation group （group A） , an ovarieetomized group （group B） , an EMF and ovariectomized group （group C） and a nilestriol and ovariectomized group （group D）. Bilateral ovariectomies were performed on all mice except those in group A. The mice of group C were exposured to a 15 Hz, 1.0 mT electromagnetic field. The mice of group D were given at nilestriol 1.5 mg/kg/week. The bone mineral density （BMD） of the lumbar vertebrae was measured before the mice were sacrificed at the 12tb week. Blood specimens were collected every two weeks to measure the ac- tivity of alkaline phosphatase （ALP） and the concentration of bone gamma-carboxyglutamic-acid-containing proteins （BGP） , calcium and estradiol in the serum. Histological sections were taken to examine and analyze the changes in bone trabeculae in the lumbar vertebrae after 6 and 1：2 weeks. Results EMF at 15 Hz and 1.0 mT intensity signifi- cantly increased the activity of ALP and the concentrations of BGP and calcium in the serum. In addition, the absorp- tion of bone trabeculae in the lumbar vertebrae was significantly restrained. Conclusions EMF at 15 Hz and 1.0 mT can restrain the development of osteoporosis in ovariectomized mice.