电针对脑梗死大鼠学习记忆能力和梗死侧海马CA3区突触结构的影响

The effects of electroacupuncture on the synaptic structure of hippocampal CA3 neurons and learning and memory ability after cerebral infarct

目的 研究电针对脑梗死大鼠学习记忆能力和梗死侧海马CA3区突触结构的影响.方法 48只Wistar雄性成年大鼠制成右侧大脑中动脉脑梗死模型后分为对照组和电针组,每组24只,2组又分别分为1,2,3周3个时段进行观察,每个时段8只.电针组于术后24 h开始进行电针治疗,对照组置于普通笼中正常喂养,不给予任何治疗.采用Morris水迷宫进行学习记忆能力评定,并观察梗死侧海马CA3区突触结构参数的变化.结果 电镜下观察到电针组各时段梗死侧海马CA3区突触后致密物厚度、活性区宽度及突触后膜曲率均较对照组明显增加,差异有统计学意义（P〈0.05）.学习记忆能力测评：对照组大鼠表现明显的学习记忆障碍,电针组在Morris水迷宫定位航行试验中和空间探索试验中均明显优于对照组（P〈0.05）.结论 电针可通过改变脑梗死大鼠梗死侧海马CA3区突触结构参数而改善脑梗死大鼠的学习记忆能力.

Objective To evaluate the effects of electroaeupuncture （EA） on synaptic interface structures around a cerebral infarct and evaluate bow learning and memory relate to synaptic structure. Methods Forty-eigbt male, adult Wistar rats were divided randomly and equally into an EA group and a control group after models of isehe- mic brain injury had been established. Each group was then divided into 1 week, 2 week and 3 week sub-groups. In the EA group EA was started 24 hours after the operation at the Baihui （DU20） and Da Zhui （BU14） points and continued for one week, two weeks or three weeks. The control group was fed in normal cages and had no special treatment. Synaptie substructures in the hippoeampal CA3 area of the ischemic side were assessed morphologically and quantitatively. Results The synaptic curvatures and postsynaptie density （PSD） as well as the width of the ac- tive area increased significantly in the EA group compared with the control group. Morris water maze learning ability in the EA group exceeded that in the control group. Spatial probe test results also significantly improved in the EA groups compared with the control groups. Conclusions Learning and memory improved in the injured rats following EA, and this is likely to be related to synaptic changes and synaptic plasticity increasing.