常熟市53起群体性流感样发热事件的实验室检测结果分析

Analysis of laboratory inspection reportsfrom 53 cases of influenza-like fever groups in Changshu

目的:探讨适合我们县级疾控开展群体性流感样发热疫情调查处理的病原学检测方法。方法:采用ELISA法和间接免疫荧光法,对流感样病例的血清样品进行甲/乙型流感病毒及其它病原体的IgM抗体检测;采用胶体金免疫色谱法,对流感样病例的咽拭子进行甲/乙型流感病毒抗原检测。结果:ELISA法检测11起疫情中62份血清样品的甲/乙型流感病毒IgM抗体,两者同时阳性38.71%(24/62);间接免疫荧光法检测13起疫情中65份血清样品的甲/乙型流感病毒IgM抗体,两者同时阳性46.15%(26/65+4/65);胶体金免疫色谱法检测44起疫情173份咽拭子的甲/乙型流感病毒抗原,甲型22.54%(39/173),乙型32.95%(57/173),两者无同时阳性。结论:我们经近六年的实际应用体会到,流感胶体金免疫色谱法明显优于ELISA法和间接免疫荧光法,适用于我们尚无条件开展病毒细胞培养和病毒核酸检测的基层疾控。

Objective：To establish a pathogen detection method for carrying out mass flu-like fever outbrecck investigation and trecctment in county-level.Methods：The virus of A/B influenza and other pathogen′s IgM anti-bodies in the serum of influenza-like cases was measured by ELISA and indirect immunofluorescence,and collaurum chromatography was also applied to test A/B influenza′s antigens in the flu-like case′s throat swabs.Results：In 62 blood serum examples tested by ELISA,which are from 11 cases,A/B influenza′s virus IgM anti-bodies appear to be positive simultaneously,38.71%（24/62）;In 65 blood serum examples coming from 13 cases,the results of indirect immunofluorescence assay was positive simultaneously with 46.15%（26/65＋4/65）,correspondingly;in 173 flu-like cases′ throat swabs measured by collaurum chromatography,A type occupies 22.54%（39/173）while B type 32.95%（57/173）.They don′t appear positive at the same time.Conclusion：According to experience about six years,It is obvious that collaurum chromatography is much better than ELISA or indirect immunofluorescence assay.Moreover,collaurum chromatography meets the need of our basic departments which lack ccnccions to carry out virus cultivation and their nucleic acid test.