南阳市2009年手足口病病原体RT-PCR检测结果分析

An analysis of test results of HFMD pathogen RT-PCR of Nanyang in 2009

目的:了解南阳市2009年3月～5月手足口病流行的主要病原体。方法:临床诊断为手足口病病例的粪便标本239份,运用逆转录聚合酶链反应(RT-PCR法)对标本病毒RNA进行肠道病毒EV71、CA16和非EV71、CA16的其他肠道病毒检测。结果:EV71阳性率为53.56%(128/239),CA16阳性率为14.23%(34/239),非EV71、CA16的其他肠道病毒阳性率为28.03%(67/239);阳性者年龄以1岁～3岁为主,男女性别比为1.73:1;发病3天内采集的标本阳性率较高;重症病例和非重症病例感染的主要病原体型别有差异。结论:引起南阳市2009年3月～5月手足口病流行的主要病原体为EV71病毒。

Objective：To learn of the main HFMD Pathogen RT-PCR in Nanyang City druing the period from March to May,2009.Methods：Select 239 stool samples of patients infected with HFMD as specimens,and then tests are conducted by the means of RT-PCR to detect such enterovirus as EV71,CA16,etc.Results：The positive rates of EV71 and CA16 are respectively 53.56%（128/239）and 14.23%（34/239）,and the positive rate of other enteroviruses is 28.03%（67/239）.Patients with positive enterovirus are mainly aged from one to three years and there are more males than females.Samples selected within three days of the disease stand good chance of being positive.There are differences between the pathogens of severe cases and non-severe cases.Conclusion：The pathogens which causes HFMD in Nanyang during the period from March to May,2009 are mainly EV71.