猪口蹄疫O型合成肽与油乳灭活疫苗免疫后抗体水平的比较

Comparison of antibody levels in response to oil-adjuvant emulsified inactivated vaccine and synthesized peptides vaccine of swine foot and mouth disease type O

为明确猪口蹄疫O型合成肽疫苗与油乳灭活疫苗免疫后的抗体水平,将65头51日龄四元杂交猪随机分为5组(13头/组),其中AA组和DD组分别为油乳灭活疫苗A及合成肽疫苗D的二次免疫组,AAA组及DDD组为相应疫苗的3次免疫组,在首免或二免后4周加强免疫,采用阻断ELISA方法检测首免后2、4、6、8、10周及12周(WPI)特异性抗体的阻断率。研究结果表明,在6 WPI,DD组及DDD组抗体阻断率开始上升,接近或达到峰值,其阻断率分别为94.5%(13/13)和97.1%(13/13),随后DD组抗体水平基本维持稳定,而DDD组抗体水平在10 WPI缓慢攀升至峰值,随后下降至65.7%(11/13);AA组和AAA组在6 WPI尽管抗体阻断率也呈上升趋势,且在10 WPI达到或接近峰值,分别为53.0%(8/13)及45.3%(6/13);综上,与猪口蹄疫O型油乳灭活疫苗2次免疫或者3次免疫相比,合成肽疫苗不仅可使机体获得较高水平的抗体,且抗体维持时间较长。

To compare the levels of antibodies against oil-adjuvant emulsified inactivated vaccine（A） and synthesized peptides vaccine（D）,sixty-five pigs of 51-day-old were randomly divided into control group,AA,DD,AAA and DDD groups that were inoculated with vaccine A or D with two or three times,and booster inoculation was conducted every 4 weeks post inoculation（WPI）.Blocking ELISA was applied to detect specific antibody blocking rates at 2,4,6,8,10 and 12 WPI,respectively.The results showed that the antibody blocking rates of inoculated group DD,and DDD peaked at 6WPI,i.e.94.5%（13/13） and 97.1%（13/13）,and the antibody levels of DD group remained stable,but the antibody levels of DDD group peaked at 10WPI,and declined to 65.7%（11/13）.Although the antibody levels of AA and AAA groups increased and peaked or nearly peaked at 10WPI,i.e.53.0%（8/13） and 45.3%（6/13）,taken together,the antibody levels responded to the synthesized peptide vaccine inoculation groups were much higher than that of the oil-adjuvant emulsified inactivated vaccine,and the corresponding lasting period of antibody levels were even longer.