新疆准噶尔铁线莲不同部位提取液抑菌活性初步研究

Antibacterial Activities of PWE and PAE from Clematis songarica Bge.in Xinjiang

用水和70%乙醇从准噶尔铁线莲不同部位中提取其活性成分,探讨其抑菌作用。结果表明:叶水提物的MIC值分别为大肠杆菌5g/100mL,金黄色葡萄球菌、短小芽孢杆菌为3g/100mL、枯草杆菌为1g/100mL;叶醇提物的MIC值分别为大肠杆菌3g/100mL,金黄色葡萄球菌、枯草杆菌为0.5g/100mL、短小芽孢杆菌1g/100mL。茎水提物的MIC值分别为大肠杆菌10g/100mL,金黄色葡萄球菌7g/100mL、短小芽孢杆菌、枯草杆菌为5g/100mL。茎醇提物的MIC值分别为大肠杆菌7g/100mL,金黄色葡萄球菌5g/100mL,枯草杆菌、短小芽孢杆菌为3g/100mL。花水提物的MIC值分别为大肠杆菌5g/100mL,金黄色葡萄球菌、枯草杆菌为3g/100mL,短小芽孢杆菌1g/100mL。花醇提物的MIC值分别为大肠杆菌、金黄色葡萄球菌、短小芽孢杆菌为1g/100mL,枯草杆菌为0.5g/100mL。果实水提物的MIC值分别为大肠杆菌、短小芽孢杆菌为5g/100mL,金黄色葡萄球菌、枯草杆菌为3g/100mL;果实醇提物的MIC值分别为大肠杆菌为3g/100mL,金黄色葡萄球菌、短小芽孢杆菌、枯草杆菌为1g/100mL。

Antibacterial activities of water extract（PWE） and alcohol extract（PAE,70g/100mL ethanol） from Clematis songarica Bge.were evaluated.Results indicated that minimum inhibition concentrations（MICs） of PWE from leaves were 5g/100mL for Echerichia coli,3g/100mL for Staphylococcus aureus and Bacillus pumilus,and 1g/100mL for Bacillus subtilis.MICs of PAE from leaves were 3g/100mL for Echerichia coli,0.5 g/100mL for Staphylococcus aureus and Bacillus subtilis,and 1 g/100mL for Bacillus pumilus.MICs of PWE from stems were 10 g/100mL for Echerichia coli,and 7 g/100mL for Staphylococcus aureus,and 5 g/100mL for Bacillus pumilus and Bacillus subtilis.MICs of PAE from stems were 7g/100mL for Echerichia coli,5 g/100mL for Staphylococcus aureus,and 3 g/100mL for Bacillus subtilis and Bacillus pumilus.MICs of PWE from flowers were 5 g/100mL for Echerichia coli,3 g/100mL for Staphylococcus aureus and Bacillus subtilis,and 1g/100mL for Bacillus pumilus.MICs of PAE from flowers were 1 g/100mL for Echerichia coli,Staphylococcus aureus and Bacillus pumilus,and 0.5 g/100mL for Bacillus subtilis.MICs of PWE from fruits were 5 g/100mL for Echerichia coli and Bacillus pumilus,and 3 g/100mL for Staphylococcus aureus and Bacillus subtilis.MICs of PAE from fruits were 3 g/100mL for Echerichia coli,and 1g/100mL for Staphylococcus aureus,Bacillus pumilus and Bacillus subtilis.