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植物乳杆菌C21胞外多糖的分离纯化及其结构修饰 被引量：3

Isolation,Purification and Structural Modification of Exopolysaccharide from Lactobacillus plantarum C21
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摘要 为研究乳酸菌胞外多糖的结构和性质,采用乙醇沉淀、DEAE-纤维素离子交换柱层析和Sepharose CL-6B凝胶过滤层析方法从植物乳杆菌(Lactobacillus plantarum)C21SDM液体培养基中分离纯化得到一种均一组分的胞外多糖,命名为PCPc。采用离子色谱和红外光谱法对其单糖组成和结构进行初步分析,结果表明,PCPc主要由半乳糖和葡萄糖两种单糖组成,其物质的量比为1:2。并对PCPc多糖进行化学修饰,获得了其硫酸化和磷酸化衍生物。 In order to investigate the structure and properties of exopolysaccharide produced by Lactobacillus plantarum C21,a neutral exopolysaccharide was isolated from culture supernatant and purified by ethanol precipitation,DEAE-cellulose ion exchange column chromatography and gel filtration chromatography on Sepharose CL-6B column.The structure of this exopolysaccharide was determined by ion chromatography and FTIR spectroscopy and this exopolysaccharide was designated as PCPc.Results indicated that the PCPc was composed of D-galactose and D-glucose in a molar ratio of 1：2.Sulfated and phosphorylated derivatives of PCPc were also prepared.
作者 李盛钰 曾宪鹏 赵玉娟 张雪 张健 杨贞耐
机构地区 吉林省农业科学院农产品加工研究中心
出处 《食品科学》 EI CAS CSCD 北大核心 2010年第9期131-134,共4页 Food Science
基金 国家自然科学基金项目(30670057)
关键词 乳酸菌 植物乳杆菌 胞外多糖 lactic acid bacteria Lactobacillus plantarum exopolysaccharide
分类号 Q939.9 [生物学—微生物学]
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参考文献14

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共引文献12

同被引文献55

  • 1牟光庆,李霞,李瑶喜,李慧.乳酸菌胞外多糖增强小鼠免疫功能的研究[J].食品科学,2009,30(5):260-262. 被引量：22
  • 2田政,王辑,郑喆,杨贞耐.乳酸菌胞外多糖的结构及功能特性研究进展[J].食品安全质量检测学报,2013,4(3):783-790. 被引量：13
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二级引证文献17

食品科学
2010年 第9期

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