影响超氧化物歧化酶活性测定的因素

Factors Affecting the Determination of Superoxide Dismutase Activity

目的:证实在黄嘌呤氧化酶法中影响超氧化物歧化酶(SOD)活性测定的干扰因素及其对保健食品抗氧化功能评价造成误差。方法:用黄嘌呤氧化酶法测定加入白蛋白、总蛋白、甘油三酯、葡萄糖、VC、还原型谷胱甘肽6种物质对SOD检测体系的影响以及小鼠灌胃VC后的SOD比活力值。结果:在黄嘌呤氧化酶法反应体系中,SOD灭活后依然能够检测到SOD比活力值。白蛋白、总蛋白、甘油三酯、葡萄糖、VC、还原型谷胱甘肽6种物质都对该体系造成干扰。透析能够消除小鼠血清中小分子物质的干扰。结论:由于黄嘌呤氧化酶法检测SOD为非特异性反应,血清中的蛋白质、脂肪、VC、还原型谷胱甘肽等物质都对SOD活性测定产生干扰而造成正误差。可采用透析的方法去除血清中含有对SOD检测体系构成干扰的小分子物质。

Objective：To verify the factors for affecting the determination of superoxide disutase（SOD） activity in xanthine oxidase method and the evaluation error for antioxidant functions of healthy food.Methods：Effects of the addition of albumin,total protein,triglyceride,glucose,vitamin C and glutathione on SOD determination in xanthine oxidase method and on specific activity of SOD in mice treated with vitamin C through gastric perfusion.Results：In xanthine oxidase method,the specific activity of SOD still could be detected although SOD was inactivated.The addition of above six substances could disturb determination results of SOD activity.However,dialysis could eliminate the interference from small molecular substances in serum.Conclusion：Xanthine oxidase method for detecting SOD activity is a non-specific method.Protein,fat,vitamin C and glutathione in serum can affect SOD activity to result in a positive error.Dialysis is a simple and effective method to remove these interference substances.