摘要
研究乳清蛋白的碱性蛋白酶水解产物对D-半乳糖(D-gal)衰老模型大鼠抗氧化效果的影响。将大鼠分为7组,包括正常对照组、D-gal模型阴性对照组、D-gal+未水解乳清蛋白组、D-gal+乳清蛋白肽低剂量组、D-gal+乳清蛋白肽中剂量组、D-gal+乳清蛋白肽高剂量组和D-gal+抗坏血酸模型阳性对照组。各处理组灌胃45d后,检测衰老大鼠血清、心脏和肾脏的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量的变化及肝脏中过氧化氢酶(CAT)活性的变化。抗坏血酸阳性对照组和低、中、高剂量乳清多肽组均可使大鼠血清、心脏和肾脏的SOD、GSH-Px及CAT活性提高,MDA含量降低,并且与阴性对照组相比差异显著(P<0.05)。其中,高剂量乳清多肽组(200mg/kgbw)对SOD酶活性作用最显著,使血清SOD活性比阴性对照组提高了29.2%。高剂量乳清多肽组使肾脏GSH-Px活性及肝脏中CAT活性达到了阳性对照抗坏血酸的水平(P>0.05),同时,中剂量乳清多肽组(100mg/kgbw)使心脏中的MDA含量与阴性对照组相比下降了38.6%。结果表明,乳清多肽能够通过提高生物体内抗氧化酶系的活力,减少自由基对组织器官的损害,发挥其抗氧化作用,这说明乳清多肽在延缓机体衰老方面具有一定的效果。
The antioxidative effect of whey protein isolate（WPI） hydrolysates on aging rats induced by D-galactose（D-gal） was investigated.The rats were divided into seven groups including the control group,D-gal model group,D-gal-induced rats treated with unhydrolyzed WPI,D-gal-induced rats treated with WPI hydrolysates at low,middle and high dosages,and D-gal-induced rats treated with ascorbic acid as the positive group.The experiment period was last for 45 days.The effect of WPI hydrolysates on antioxidative enzyme system including superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） and hydrogen peroxidase（CAT） in rats was evaluated,and the content of malondialdehyde（MDA） was determined.Results showed that WPI hydrolysates at three dosage levels significantly increased SOD and GSH-Px activities and simultaneously decreased MDA content in serum,heart and kidney tissues.The CAT activity in liver tissue of treated rats was also improved（P 0.05）.Moreover,a higher SOD activity was observed in rats treated with WPI hydrolysates at the dosage of 200 mg/kg· bw.Compared with the negative control,the SOD activity in serum of rats treated with WPI hydrolysates at the high dosage was increased by 29.2%.No significant difference in GSH-Px activity in kidney and CAT activity in liver between WPI hydrolysate treatment at the high dosage and the positive control of ascorbic acid was observed（P 0.05）.In contrast,compared with the negative control,the MDA content in rats treated with WPI hydrolysates at the dosage of 100 mg/kg bw was decreased by 38.6% in heart.Therefore,WPI hydrolysates delayed the aging process of rats due to the increase of the activity in antioxdative enzyme system.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第9期238-242,共5页
Food Science
基金
国家“863”计划项目(2008AA10Z315)
