超微化豆渣膳食纤维对BALB/c小鼠血清胆固醇及甘油三酯的影响

Effect of Ultra-micronized Okara Dietary Fiber on Serum Cholesterol and Triglyceride Levels in BALB/c Mice

以豆渣为原料提取豆渣膳食纤维(ODF),采用超微粉碎干法粉碎及动态高压微射流进行湿法粉碎分别得到超微粉碎处理的膳食纤维(D-ODF)、微射流处理的膳食纤维(W-ODF),未经超微化处理的样品作为对照(C-ODF)。观察超微化前后膳食纤维的主要理化性质的变化,并研究其对BALB/c小鼠血清中总胆固醇(TC)、高密度胆固醇(HDL-C)及甘油三酯(TG)水平的影响。结果表明,W-ODF样品的粒度比D-ODF组降低更为显著,且膨胀力、持水力及可溶性膳食纤维含量均高于D-ODF及C-ODF。灌胃28d后,D-ODF组小鼠的TC水平较灌胃前有所下降,对照组及W-ODF组TC水平有所升高,但各组HDL-C的水平均有所升高;W-ODF组中HDL-C/TC提高了21.2%,显著高于C-ODF(4.8%)和D-ODF组(11.4%);对照组TG水平较灌胃前有所升高,而D-ODF组和W-ODF组分别降低了13.5%和29.2%;D-ODF、W-ODF组的TG水平较对照组分别降低了17.9%(P<0.05)、41.0%(P<0.05),后者效果更为显著。

Dietary fiber was separated from okara,and then micronized through dry grinding method using ultra-micro pulverizer and wet granulating method using dynamic high pressure microfluidization,respectively.The samples were collected as D-ODF and W-ODF.Un-micronized dietary fiber（C-ODF） was the control diet.The changes of major physical and chemical properties before and after micronization were examined and effects of these samples on levels of serum total cholesterol（TC）,high-density lipoprotein cholesterol（HDL-C） and triglyceride（TG） were determined in BALB/c mice.Results indicated that particle size of W-ODF exhibited more significant declined than that of D-ODF.Meanwhile,swelling capacity,water holding capacity and soluble dietary fiber content in W-ODF were higher than those of D-ODF and C-ODF.The TC level exhibited a decrease in mice fed with D-ODF for 28 days through comparing with the TC level pre-treatments.However,the TC level in mice of both control groups and W-ODF group revealed an increase.In addition,serum HDL-C levels of all groups exhibited an increase;the ratio of HDLC/TC in W-ODF group was increased by 21.2%,which was significantly higher than that of C-ODF（4.8%） and D-ODF（11.4%）.TG level of control group was also increased,however,13.5% reduction in D-ODF group and 29.2% decrease in W-ODF group were observed,compared with pre-feeding.Compared with the control group,TG levels in D-ODF group and W-ODF group were decreased by 17.9%（P 0.05） and 41.0%（P 0.05）,respectively.W-ODF exhibited more prominent effect on TG level.