红菇多糖的提取及其降血糖、血脂作用研究

Polysaccharide Extract from Russula and Its Role of Lowering Blood Glucose and Lipid

目的:探讨红菇多糖的提取纯化及其降血糖、血脂作用。方法:用热水浸提法从红菇子实体提取粗多糖,并进行离子交换层析及凝胶层析纯化。通过注射四氧嘧啶和饲喂高脂饲料建立糖尿病及高脂血症小鼠模型,然后用红菇多糖给药处理,检测血糖、血脂各项指标的变化情况。结果:与对照组相比,红菇多糖给药组的葡萄糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)指标极显著下降(P<0.01),高密度脂蛋白胆固醇(HDL-C)指标极显著上升(P<0.01),并且高剂量组比低剂量组效果更好。结论:红菇多糖有降血糖、血脂作用。

Objective：To explore the purification of Russula polysaccharide and its role of lowering glucose and lipid.Methods：Crude polysaccharide was extracted from Russula fruit bodies through soaking extraction method in hot water and purified through ion-exchange chromatography and gel chromatography.The mice model with diabetes and hyperlipidemia was established through the injection of alloxan and the feeding of high-fat food.The model mice were administrated with Russula polysaccharide.Blood glucose and blood lipid in model mice with diabetes and hyperlipidemia were evaluated.Results：GLU,TC,TG and LDL-C exhibited a significant decrease（P 0.01） in mice with the administration of Russula polysaccharide in a dose-dependent manner.Conclusion：Russula polysaccharide has excellent functions in lowering blood glucose and lipid levels.