黄连解毒汤半仿生提取法提取药材组合方式的优选

Optimization of combination modes of medical materials in extract of Huanglian Jiedu decoction by semi-bionic extraction

目的:优选黄连解毒汤药效物质提取时方药最佳组合方式。方法:将方药排列组合成15组,用半仿生提取法提取;以小檗碱、黄芩素、栀子苷、总生物碱、总黄酮、HPLC总面积、≤1 000提取物为指标,将各指标测得值进行标准化处理,并经加权求和后得综合评价Y值,Y值大者为优。结果:7个指标综合评价Y值以AD+BC最大。结论:黄连解毒汤的提取,以黄连和栀子、黄芩和黄柏分别合提,再将提取液合并为最佳。

Objective： To optimize the combination modes of medical materials in extract of Huanglian Jiedu decoction.Method： Divided the medical materials of Huanglian Jiedu decotion into 15 groups and extracted by semi-bionic estraction（SBE）,the contents of berberine,baicalin,geniposide,total alkaloid,total flavonoid,total integral calculus area of HPLC and extract of 1 000 were determined,standardized,weighted and evaluated,respectively.Result： The maximum evaluated value Y for the extracting technique was AD＋BC.Conclusion： The optimal combination mode of the perscription is that Rhizoma Coptidis and Fructus Gardeniae,Radix Scutellariae and Cortex Phellodendri Amurensis are decocted together respectively then the two decoctions are merged.