柑橘皮总黄酮对大鼠佐剂性关节炎的影响及机制研究

Effect and mechanism of total flavonoids of orange peel on rat adjuvant arthritis

目的:探讨柑橘皮总黄酮(TFO)对大鼠佐剂性关节炎(AA)发展的影响及机制。方法:建立大鼠AA模型,灌胃给药37.5,75,150 mg.kg-1TFO。游标卡尺测量大鼠足跖厚度,HE染色观察关节病理损伤,放射免疫法检测血清肿瘤坏死因子(TNF)-α、白介素(IL)-1β和前列腺素(PG)E2水平,Western blot法分析关节滑膜组织环加氧酶(COX)-2表达。结果:75,150 mg.kg-1TFO可显著减少AA大鼠足跖厚度,减轻关节病理学损伤,降低血清TNF-α,IL-1β,PGE2水平,抑制关节滑膜组织COX-2表达。结论:TFO可拮抗大鼠AA发展,其作用机制与抑制炎症介质的生成密切相关。

Objective： To investigate the effect of the total flavonoids of orange peel（TFO） against adjuvant arthritis（AA） and the underlying mechanism.Method： AA model was induced in male SD rats by immunization with Freund＇s complete adjuvant.Pad thickness was assayed by caliper.Pathological impairment of ankle joint was analysised by hematoxylin and eosin（HE） staining.Levels of tumor necrosis factor（TNF）-α,Interleukin（IL）-1β and prostaglandin（PG）E2 in serum was detected by radioimmunoassay method.Cyclooxygenase（COX）-2 expression in synovium tissues was measured by Western blot assay.Result： The 75 mg·kg-1 and 150 mg·kg-1 TFO treatment obviously decreased the pad thickness and improve the pathological impairment of ankle joint of AA rats.In addition,abnormal elevation of TNF-α,IL-1β and PGE2 in serum and COX-2 expression in synovium tissues of AA rats were markedly repressed by TFO treatment.Conclusion： TFO can inhibit the development of AA in rats,and the mechanism were likely due to depressing inflammatory mediators production.