茶多酚及其主要成分EGCG在辐射损伤小鼠模型中的保护作用

Protective activity of different concentration of tea polyphenols and its major compound EGCG against whole body irradiation-induced injury in mice

目的:评价不同含量茶多酚(TP)及其主要成分表没食子儿茶素没食子酸酯(EGCG)的抗辐射及抗氧化作用。方法:采用60Coγ射线照射小鼠造成亚急性损伤模型,给予不同剂量TP及EGCG后1,3,7,14,21,28 d检测对小鼠外周血指标和脏器系数的影响,同时检测血清中抗氧化酶(SOD)和脂质过氧化物(MDA)水平。结果:与非照射组相比,辐射组可明显降低脾腺指数,降低外周血白细胞(WBC)、红细胞(RBC)和血小板(PLT);同时降低过氧化物歧化酶并升高脂质过氧化物水平。给予TP(50 mg.kg-1)及EGCG(20 mg.kg-1)治疗后,可明显缓解由于辐射损伤所造成的全血象下降,且表现出较好的体内抗氧化活性。结论:含50%EGCG的茶多酚(TP50)具有明显缓解辐射所造成的造血功能损伤和抗氧化作用。

Objective： To evaluate the different concentrate of tea polyphenols（TP） and its compound for irradiation-protection and investigate its mechanism.Method： To evaluate the radioprotective activity,mice were exposed to whole body gamma irradiation.TP 80 and TP 50（50,10 mg·kg-1） and its major constituent epigallocatechin gallate（EGCG）（50,10 mg·kg-1） were administered after irradiation to examine its inhibition against irradiation-induced injury.Result： This study indicate that in comparison with non-irradiated controls,irradiation resulted in a significant reduction the spleen index（spleen weight/body weight 100）,haematological parameters（RBC,WBC and PLT）,activity of superoxide dismutase（SOD）,and increase of malondialdehde（MDA） level in 28 days.Oral administration of TP（50 mg·kg-1） shown the best effect on reducing the irradiation-induced injury on mice studied,and showed a protective effect against irradiation-induced haematological parameters（RBC,WBC and PLT）,the spleen index and MDA level significant reduction,and antioxidase activity（SOD） decrease.Conclusion： The results suggest that TP 50 mg·kg-1 and EGCG have in vivo antioxidant potential and radioprotective activity against whole body gamma irradiation in mice.It may be concluded that TP（50% EGCG） possess good irradiation-protective and antioxidant effect.[