清营泻瘀方对肠缺血再灌注损伤大鼠的保护作用及机制

Experiment study of Qing-ying-xie-yu Fang (QXF) in the treatment of intestinal ischemia-reperfusion in rats

目的:探讨清营泻瘀方对肠缺血再灌注损伤大鼠的保护作用及可能机制。方法:40只SD大鼠,随机分为假手术组(n=8)、肠缺血再灌注模型组(n=16)和清营泻瘀方治疗组(n=16),后2组按造模后处死时间的不同又分别分为术后2h组和术后24h组,每组8只。采用夹闭肠系膜上动脉45min的方法诱导肠缺血再灌注损伤模型,分别观察再灌注后2h和24h肠黏膜的病理改变以及血清中TNF-α、IL-10和血浆中D-乳酸的水平。结果:通过夹闭肠系膜上动脉45min的方法成功诱导出肠缺血再灌注损伤模型,清营泻瘀方治疗组能够减轻肠黏膜的损伤,降低血中TNF-α和D-乳酸的含量(P<0.05),晚期降低IL-10水平(P<0.05)。结论:清营泻瘀方对肠缺血再灌注损伤大鼠有显著的保护作用,其机制可能与荡涤肠胃宿垢、减少细菌移位、调节炎症介质平衡和保护肠屏障有关。

AIM：To investigate the protective effects and possible mechanisms of Qing-ying-xie-yu Fang （QXF） in the treatment of intestinal ischemia-reperfusion in rats. METHODS： Forty Sprague Dawley rats were randomly divided into sham（n=8）, model（n= 16） and QXF（n= 16） groups. The last two groups were divided into 2 hours group and 24 hours group after reperfusion respectively. Intestinal ischemia reperfusion model in rats was induced by clamping the superior mensenteric artery＇ for 45 minutes. The pathologic injury of the intestines and the levels of TNF-α,IL-10,D lactic acid in blood were examined at 2 hours point reperfusion respectively. and 24 hours point after The statistical analysis was finished with SPSS 11. 0 software. RESULTS： Intestinal ischemia-reperfusion model in rats was successfully induced by clamping the superior mensenteric artery for 45 minutes. QXF alleviated the pathologic injury of the intestines, and reduced the levels of TNF-α, D lactic acid （P〈0.05） in the early period and the level of IL-10 （P〈0.05） in the late period. CONCLUSION： QXF can protect the intestines of rats from the injury of intestinal ischemia-reperfusion. The following is the possible mechanisms-to clear up dunghill in intestines, regulate the balance of inflammatory mediators and protect gut barrier function.