体外培养的人鼻腔黏膜纤毛的摆动频率

The ciliary beat frequency of human nasal epithelium in vitro

目的通过测量体外培养的人鼻腔纤毛上皮细胞的纤毛摆动频率(ciliray beat frequency,CBF),为人鼻腔黏液纤毛运输系统功能的研究提供理论依据。方法应用组织块培养法和高速数字化摄像系统,测量体外培养的人鼻腔钩突、下鼻甲黏膜的CBF。结果体外培养的正常鼻腔黏膜上皮细胞和鼻窦炎鼻腔黏膜上皮细胞的CBF,两者之间差异无统计学意义(t=0.419,P=0.678);测量来源相同体外培养的钩突和下鼻甲纤毛上皮细胞的CBF,两者的CBF是一致的,之间的差异无统计学意义(t=-0.087,P=0.931)。结论体外培养的鼻窦炎患者的鼻腔黏膜可代表正常人的鼻腔黏膜进行黏液纤毛运动的研究;钩突黏膜更容易获得,可代替下鼻甲黏膜进行体外培养。

OBJECTIVE To explore the valuable information for studying human nasal mucociliary transport system by measuring the nasal ciliary beat frequency（CBF）in vitro.METHODS CBF of cultured human nasal epithelial cells was measured by highspeed digital microscopy in HBSS.RESULTS CBF of nasal epithelial cells from inferior turbinate and uncinate process of same patient has no significant difference in vitro（t=-0.087,P=0.931）.CBF of normal mucosa and uncinate process from patients of sinusitis has no significant difference in vitro（t=0.419,P=0.678）.CONCLUSION CBF of nasal mucosa of sinusitis can replace the CBF of normal nasal mucosa in vitro for for nasal ciliary movement studying.Because the mucosa of uncinate process is easier to obtain,it can replace the mucosa of inferior turbinate for cell culture in vitro.