子宫内膜癌组织中Id-1、VEGF-C和EGFR的表达及临床意义

Expression of Id-1,VEGF-C and EGFR in endometrial adenocarcinomas and their significance

目的探讨Id-1、VEGF-C和EGFR在正常子宫内膜、子宫内膜增殖症和内膜癌组织中的表达及临床病理学意义。方法采用组织芯片技术和免疫组化检测30例正常子宫内膜组织、30例单纯性增生过长、30例复杂性增生伴不典型增生、72例子宫内膜癌组织中Id-1、VEGF-C和EGFR蛋白表达。结果Id-1、VEGF-C和EGFR蛋白在子宫内膜腺癌组织中的阳性表达率明显高于正常子宫内膜和子宫内膜增殖症(P<0.01),Id-1过表达与组织学分级、临床分期、浸润程度和淋巴结转移有关(P<0.01,P<0.01,P<0.05,P<0.05);VEGF-C和EGFR阳性表达均与临床分期、浸润程度和淋巴结转移有关(P<0.05),与组织学分级无关;Id-1与VEGF-C和EGFR蛋白表达呈正相关(rs=0.625,P<0.01;rs=0.313,P<0.008)。结论Id-1蛋白参与VEGF-C和EGFR调控,指示其可能与子宫内膜癌的发生发展有关,联合检测可作为判断子宫内膜癌浸润和转移的重要指标。

Purpose To investigate the relationship of the expression of Id-1,VEGF-C and EGFR proteins in the development of endometrial carcinoma.Methods The expression levels of Id-1,VEGF-C and EGFR proteins in endometrial adenocarcinoma tissues（n=72）,endometrial hyperplasia tissues（n=60） and normal tissues（n=30） were examined by tissue microarray technique and immunohistochemistry（MaxVision method）.Results The positive expression rates of Id-1,VEGF-C and EGFR proteins in normal endometrium,endometrial hyperplasia,endometrial atypical hyperplasia and endometrial carcinomas were gradually increased（P0.01）.The positive expression of Id-1 was related to the histological grade（P0.01）,FIGO stage（P0.01）,depth of invasion（P0.05） and lymph node metastasis（P0.05）.The positive expression of VEGF-C and EGFR was related to FIGO stage（P0.05）,depth of invasion（P0.05） and lymph node metastasis（P0.05） but it was not related to the histological grade.The expression of Id-1 protein was positively related to that of VEGF-C and EGFR（r=0.625,P=0.000;r=0.313,P=0.008）.Conclusion This study reveals that Id-1,VEGF-C and EGFR takes part in development and progression of endometrial adenocarcinomas and that Id-1 may associate with regulations of VEGF-C and EGFR.The results of this study support the idea that not only VEGF-C and EGFR but also Id-1 may be important biomarkers in the detection of metastasis and biological behavior in endometrial carcinoma.