口腔疣状癌与口腔鳞癌中PTTG1 mRNA表达的对比研究

The Expression of PTTG1 in oral verrucous carcinoma and oral squamous cell carcinoma

目的:研究垂体肿瘤转化基因-1(Pituitary tumor transforming gene 1,PTTG1)在口腔疣状癌中的表达,并与口腔鳞癌(OSCC)作比较,探讨其在口腔疣状癌及OSCC中发生发展中的作用。方法采用逆转录多聚酶链反应(RT-PCR)技术分别检测口腔疣状癌和OSCC中PTTG1 mRNA相对含量,并取自身的癌旁黏膜和正常口腔黏膜作比较。结果:PTTG1在疣状癌和无淋巴结转移的高分化SCC的癌组织、正常黏膜和癌旁黏膜中均有不同程度的表达,但这种表达无显著差异(P>0.05);在有淋巴结转移的高分化SCC中,PTTG1的表达在癌组织和癌旁黏膜比正常黏膜有增高的趋势,中分化SCC中这种增高趋势更为明显,但统计学上均无显著意义(P>0.05)。PTTG1在口腔疣状癌、无淋巴结转移的高分化SCC、有淋巴结转移的高分化SCC及中分化SCC四组癌组织中的表达呈现依次增加的趋势,其中口腔疣状癌的表达低于中分化SCC(P<0.05),而与高分化SCC无差别(P>0.05)。结论:在口腔疣状癌,PTTG1的表达与高分化SCC一致,但低于中分化SCC,从总体上说明口腔疣状癌的生物学行为比中分化SCC好,而与高分化SCC一致;PTTG1与口腔疣状癌的发生可能无关,但可能参与了SCC的发生发展过程。

Objective：to investigate the expression levels of PTTG1 in human oral verrucous carcinoma（OVC）and oral squamous cell carcinoma（SCC）tissues as well as in the paracancerous mucous（P）and nomal mucous（N）of the patient with OVC or SCC.Method：Expression of PTTG1 messenger ribonucleic acid（mRNA）was evaluated by reverse transcription polymerase chain reaction（RT-PCR）in oral verrucous carcinoma（OVC）,oral squamous cell carcinoma（SCC）,normal mucous and paracancerous mucous of the patient with OVC or SCC.The data was analyzed with reference to clinicopathological factors.Result：Tumor tissue and paracancerrous mocous and nomal mucous of OVC had no difference（P 0.05）;tumor tissue and paracancerrous mocous and nomal mucous of SCC had also no difference in statistics（P 0.05）,but the expression of PTTG1 in tumor tissue had a upward trendency.The expression of PTTG1 existed upward trendency,one by one,in tumor tissue of OVC,wdSCC,wdSCC-L and mdSCC.OVC had a significantly lower level of PTTG1 than mdSCC（P 0.05）,but had no difference with wdSCC（P 0.05）.Conclusion：In OVC,the expression of PTTG1 consistented with wdSCC,and had a lower level than mdSCC,It is suggested that OVC resembled wdSCC,but not mdSCC;Expression of PTTG1 had no difference among tumor tissue,normal mocous and paracancerrous mocous of OVC,but in SCC,tumor tissue had a upward trendency,It is suggested that PTTG1 may associated with tumorgenesis and metastasis of SCC,but not OVC.