TAP-1等位基因多态性与复发性尖锐湿疣间的关系

Relationship of TAP-1 allele gene polymorphism to recurrent condyloma acuminatum

目的 探讨TAP-1333、TAP-1637基因多态性与中国人群复发性尖锐湿疣（CA）的关系.方法 采用扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）技术检测88例复发性CA患者和81例正常人对照者TAP-1333基因位点的A/G单核苷酸多态性,以及60例复发性CA患者和60例健康对照者（对照组）TAP-1637基因位点的A/G单核苷酸多态性.结果 CA组TAP-1333等位基因AA、GG、AG基因频率分别为86.36%、0%和13.64%,对照组各等位基因频率分别为79.01%、0%和20.99%,组间差异无统计学意义（χ2=1.604,P＞0.05）,OR值=1.682（0.748～3.783）.CA组TAP-1637等位基因AA、GG、AG基因频率分别为3.33%、95.00%和1.67%,而对照组各等位基因频率分别为10.00%、60.00%和30.00%,在CA组,天冬氨酸（Asp）杂合型和纯合型出现频率明显低于对照组,而甘氨酸纯合型明显高于对照组,组间差异有统计学意义（χ^2=23.682,P＜0.01）.AA型：OR值=0.310（0.060～1.604） GG型：OR值=12.667（3.555～45.135） AG型：OR值=0.04（0.005～0.308）.结论 TAP-1333位点基因多态性与复发性CA无关 TAP-1637基因位点多态性可能与复发性CA有相关性.

Objective To investigate the relationship of polymorphisms at codons 333 and 637 of TAP-1 allele gene to recurrent condyloma acuminatum （CA） in a Chinese population. Methods Amplificatory refraction mutation system-PCR （ARMS-PCR） was performed to detect TAP1 polymorphic residues at codon 333 in 88 patients with recurrent CA and 81 age- and sampling date-matched controls and at codon 637 in 60 patients with recurrent CA and 60 age- and sampling date-matched controls. Results The frequencies of AA,GG and AG genotypes at codon 333 of TAP-1 gene were 86.36%, 0, 13.64%, respectively, in patients with recurrent CA, 79.01%, 0 and 20.99%, respectively, in the controls, and no significant difference was observed between the two groups （χ^2 = 1.604, P 〉 0.05）. However, a significant difference was observed in the frequencies of AA, GG and AG genotypes at codon 637 of TAP-1 gene between the patients and controls （3.33% vs 10.0%, 95.00% vs 60.00%, 1.67% vs 30.00%, χ^2 = 21.551, P 〈 0.01 ）. Conclusions The recurrence of CA may be associated with the polymorphism at codon 637, but not with that at codon 333, of TAP-1 gene.