中药单体氢溴酸槟榔碱对糖尿病胃轻瘫大鼠胃动力的影响

Traditional Chinese medicine monomer arecoline promotes gastric motility in diabetic gastroparesis rats

目的:应用放射性核素单光子发射断层显像技术胃排空测定法(SPECT)观察中药单体氢溴酸槟榔碱(ARE)对糖尿病胃轻瘫(DGP)大鼠胃动力的影响及可能的作用机制.方法:清洁级(SPF)♂Wistar大鼠95只,随机分为正常对照组(n=20)、实验组(n=75).正常对照组以普通饲料喂养20wk,实验组以高脂高糖饲料喂养6wk后,小剂量链脲佐菌素(STZ)腹腔注射建立糖尿病模型,以高糖高脂饲料及饮食失节法继续诱导20wk后,应用SPECT筛检出DGP大鼠模型,随机分为模型组、氢溴酸槟榔组(ARE)及红霉素组(ERY),而后进行药物尾静脉注射,前后再次测定胃排空.实验结束时各组大鼠空腹12h(禁食不禁水)后采血用生物化学方法测定血脂、血糖.结果:正常对照组、DGP模型组胃半排空时间(GET1/2)分别为71.96min±14.60min、161.9min±10.61min,模型组GET1/2显著高于正常对照组,差异有统计学意义(P<0.05),ARE组大鼠治疗后GET1/2(165.8min±18.30min)缩短为99.6min±16.60min,ERY组大鼠治疗后GET1/2(164.1min±20.71min)缩短为133.3min±19.70min,与本组治疗前比较均显著缩短,差异有统计学意义(P<0.05).ARE组GET1/2较ERY组缩短显著,组间比较差异有统计学意义(P<0.05),模型组治疗前后比较差异无统计学意义.实验结束时模型组大鼠空腹血糖(GLU)、三酰甘油(TC)较正常对照组明显升高,ARE组及ERY组治疗后TG较模型组下降,差异有统计学意义(P<0.05),GLU、TC、LDL-C均有下降趋势,差异无统计学意义.结论:ARE及ERY均可促进DGP大鼠的GET1/2,其中ARE的治疗效果优于ERY;给予ARE及ERY治疗后TG水平下降.

AIM：To investigate the effects of traditional Chinese medicine （TCM） monomer arecoline （ARE） on gastric emptying in diabetic gastroparesis （DGP） rats by single-photon emission computed tomography （SPECT）.METHODS：Ninety-five specific pathogen-free male Wistar rats were divided randomly intotwo groups：control group （n = 20） and experi-mental group （n = 75）. The control group was fed a normal diet for 20 wk,while the experi-mental group was fed a fat-and caloric-rich diet for the same duration and injected with small dose of streptozotocin （STZ） at week 6 to induce diabetes. Gastric emptying half-time （GET1/2） was measured by SPECT. DGP rats were then subdivided into three groups：model group （n = 20）,ARE group （n = 20） and erythromycin （ERY） group （n = 20）. These three groups were intravenously injected with normal saline,ARE and erythromycin,respectively. GET1/2 was measured again. Blood glucose （GLU）,total cho-lesterol （TC）,triglyceride （TG）,high-density li-poprotein cholesterol （HDL-C）,and low-density lipoprotein cholesterol （LDL-C） were measured using biochemical method.RESULTS：The GET1/2 was signifi cantly longer in the DGP model group than in the normal control group （161.90 min ± 10.61 min vs 71.96 min ± 14.60 min,P〈0.05）. Treatment with both ARE and ERY significantly shortened GET1/2 （99.60 min ± 16.60 min vs 165.80 min ± 18.30 min and 133.30 min ± 19.70 min vs 164.10 min ± 20.71 min,both P〈0.05）. The shortened GET1/2 was more significant in the ARE group than in the ERY group （P〈0.05）. At the end of the experi-ment,fasting GLU and TG levels were signifi-cantly higher in the model group than in the normal control group. In contrast,TG level was significantly lower in the ARE group and ERY group than in the model group （P〈0.05）.CONCLUSION：Both ARE and ERY can promote gastric emptying in DGP rats. ARE is superior to ERY in promoting gastric emptying in DGP rats. Treatment with both ERY and ARE reduces blood TG level.