干细胞因子对糖尿病小鼠小肠平滑肌细胞慢波的影响

Effects of stem cell factor on the slow waves in intestinal smooth muscle of diabetic mice

目的:探讨外源性干细胞因子(stem cell factor,SCF)对糖尿病(diabetes mellitus,DM)小鼠小肠动力障碍时小肠平滑肌细胞慢波的影响.方法:♂Balb/c小鼠一次性腹腔注射(ip)链脲佐菌素(STZ,150mg/kg)造模,将小鼠分为正常组、DM组、DM+外源性SCF组(DM+SCF组);DM+SCF组ipSCF0.20μg/(kgd),正常组和DM组每天ip等量的磷酸盐缓冲液(pH7.40).所有小鼠干预6wk结束后,给予印度墨水灌胃测定小肠传输速率,用微电极细胞内记录仪记录各组小鼠十二指肠平滑肌细胞内慢波的变化.结果:DM组小肠推进率比正常组的明显降低(44.05%±5.48%vs82.75%±6.56%,P<0.01);DM+SCF组比DM组的小肠推进率显著增加(75.89%±3.61%vs44.05%±5.48%,P<0.01),但比正常组的降低(75.89%±3.619%vs82.75%±6.56%,P<0.05);DM组与正常小鼠相比,十二指肠平滑肌细胞内慢波频率明显减慢(13.33±4.27vs30.67±3.33,P<0.01),波幅明显减小(15.17±3.71vs35.17±3.71,P<0.01),且波形杂乱不规则.DM+SCF组小鼠十二指肠平滑肌细胞内慢波频率和波幅比DM组增加(26.50±1.87vs13.33±4.27;27.50±2.26vs15.17±3.71,均P<0.01),但比正常组减慢和降低(26.50±1.87vs30.67±3.33,P<0.05;27.50±2.26vs35.17±3.71,P<0.01).结论:外源性SCF对DM小鼠的小肠动力障碍有一定的改善作用.

AIM：To explore the effects of stem cell factor （SCF） on the slow waves in duodenal smooth muscle of diabetic mice.METHODS：Diabetes mellitus （DM） was induced in Balb/c male mice by intraperitoneal injection of streptozotocin （STZ,150 mg/kg,ip）.Mice were then divided into three groups （n=6 per group）：normal control group,DM model group and SCF treatment group.The SCF treatment group was given SCF 0.20 μg/（kg· d,ip）,while the normal control group and DM model group were given equal volume of phosphate buffer solution （pH7.40）.After six weeks of intervention,all the mice were given Indian inkfor determination of the small intestinal transit rate.The changes in the slow waves in duodenal smooth muscle were recorded.RESULTS：Small intestine transit rate was significantly reduced in the DM model group when compared with the normal control group （44.05% ± 5.48% vs 82.75% ± 6.56%,P〈0.01）.Small intestine transit rate in the SCF treatment group was significantly higher than that in the DM model group （75.89% ± 3.61% vs 44.05% ± 5.48%,P〈0.01）,but lower than that in the normal control group （75.89% ± 3.61% vs 82.75% ± 6.56%,P〈0.05）.Compared with the normal control group,the frequency and amplitude of slow waves in duodenal smooth muscle were significantly reduced in the DM model group （13.33 ± 4.27 vs 30.67 ± 3.33 and 5.17 ± 3.71 vs 35.17 ± 3.71,respectively;both P〈0.01）.The frequency and amplitude of slow waves in duodenal smooth muscle in the SCF treatment group were significantly higher than those in the DM model group （26.50 ± 1.87 vs 13.33 ± 4.27 and 27.50 ± 2.26 vs 15.17 ± 3.71,respectively;both P〈0.01）,but lower than those in the normal control group （26.50 ± 1.87 vs 30.67 ± 3.33,P〈0.05;27.50 ± 2.26 vs 35.17 ± 3.71,P〈0.01）.CONCLUSION：Exogenous SCF is able to improve duodenal motility in diabetic mice.